Two-Color Probe to Monitor a Wide Range of pH Values in Cells†
Min Hee Lee
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorJi Hye Han
The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701 (Korea)
Search for more papers by this authorJae Hong Lee
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorNayoung Park
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorRajesh Kumar
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorCorresponding Author
Prof. Chulhun Kang
The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701 (Korea)
Chulhun Kang, The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701 (Korea)
Jong Seung Kim, Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorCorresponding Author
Prof. Jong Seung Kim
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Chulhun Kang, The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701 (Korea)
Jong Seung Kim, Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorMin Hee Lee
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorJi Hye Han
The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701 (Korea)
Search for more papers by this authorJae Hong Lee
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorNayoung Park
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorRajesh Kumar
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorCorresponding Author
Prof. Chulhun Kang
The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701 (Korea)
Chulhun Kang, The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701 (Korea)
Jong Seung Kim, Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorCorresponding Author
Prof. Jong Seung Kim
Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Chulhun Kang, The School of East-West Medical Science, Kyung Hee University, Yongin, 446-701 (Korea)
Jong Seung Kim, Department of Chemistry, Korea University, Seoul, 136-701 (Korea)
Search for more papers by this authorThis work was supported by the CRI project (20120000243, J.S.K.) and by the Basic Science Research Program (2012R1A1A2006259, C.K.) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology.
Graphical Abstract
High-tech pH paper: A “chameleon” pH probe composed of rhodamine (red, see scheme) and fluorescein (green) units emits at wavelengths of 580 nm and 512 nm, where the intensities show a contrary response to pH changes. Confocal microscopy of HeLa cells with this probe reveals red and green spots; the ratio of these signals can be calibrated to give the pH value of the respective organelle.
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