NMR Spectroscopy of Soluble Protein Complexes at One Mega-Dalton and Beyond†
Dr. Andi Mainz
Munich Center for Integrated Protein Science (CIPS-M) at the Department Chemie, Technische Universität München (TUM), Lichtenbergstrasse 4, 85747 Garching (Germany)
Helmholtz-Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt, Neuherberg (Germany)
Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin (Germany)
Search for more papers by this authorProf. Dr. Tomasz L. Religa
University of Toronto, Department of Medical Genetics and Microbiology (Canada)
Search for more papers by this authorDr. Remco Sprangers
Max Planck-Institute for Developmental Biology, Tübingen (Germany)
Search for more papers by this authorDr. Rasmus Linser
Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston (USA)
School of Chemistry, UNSW, Sydney (Australia)
Search for more papers by this authorProf. Dr. Lewis E. Kay
University of Toronto, Department of Medical Genetics and Microbiology (Canada)
Program in Molecular Structure and Function, Hospital for Sick Children, Toronto (Canada)
Search for more papers by this authorCorresponding Author
Prof. Dr. Bernd Reif
Munich Center for Integrated Protein Science (CIPS-M) at the Department Chemie, Technische Universität München (TUM), Lichtenbergstrasse 4, 85747 Garching (Germany)
Helmholtz-Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt, Neuherberg (Germany)
Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin (Germany)
Munich Center for Integrated Protein Science (CIPS-M) at the Department Chemie, Technische Universität München (TUM), Lichtenbergstrasse 4, 85747 Garching (Germany)Search for more papers by this authorDr. Andi Mainz
Munich Center for Integrated Protein Science (CIPS-M) at the Department Chemie, Technische Universität München (TUM), Lichtenbergstrasse 4, 85747 Garching (Germany)
Helmholtz-Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt, Neuherberg (Germany)
Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin (Germany)
Search for more papers by this authorProf. Dr. Tomasz L. Religa
University of Toronto, Department of Medical Genetics and Microbiology (Canada)
Search for more papers by this authorDr. Remco Sprangers
Max Planck-Institute for Developmental Biology, Tübingen (Germany)
Search for more papers by this authorDr. Rasmus Linser
Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston (USA)
School of Chemistry, UNSW, Sydney (Australia)
Search for more papers by this authorProf. Dr. Lewis E. Kay
University of Toronto, Department of Medical Genetics and Microbiology (Canada)
Program in Molecular Structure and Function, Hospital for Sick Children, Toronto (Canada)
Search for more papers by this authorCorresponding Author
Prof. Dr. Bernd Reif
Munich Center for Integrated Protein Science (CIPS-M) at the Department Chemie, Technische Universität München (TUM), Lichtenbergstrasse 4, 85747 Garching (Germany)
Helmholtz-Zentrum München (HMGU), Deutsches Forschungszentrum für Gesundheit und Umwelt, Neuherberg (Germany)
Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin (Germany)
Munich Center for Integrated Protein Science (CIPS-M) at the Department Chemie, Technische Universität München (TUM), Lichtenbergstrasse 4, 85747 Garching (Germany)Search for more papers by this authorThis work was performed in the framework of SFB-1035/Project-B07 (German Research Foundation, DFG). We acknowledge support by the Leibniz-Gemeinschaft, the Helmholtz-Gemeinschaft, and the Deutsche Forschungsgemeinschaft (Grants Re1435). We are also grateful to the Center for Integrated Protein Science Munich (CIPS-M) for financial support (A.M., B.R.). This work was further supported by the Max Planck Society (R.S.) and by a grant from the Canadian Institutes of Health Research (CIHR) (T.L.R., L.E.K.). L.E.K. holds a Canada Research Chair in Biochemistry. R.L. was supported by an Australian Research Council Discovery Early Career Research Award.
Graphical Abstract
Bigger is better: Sequential backbone assignments are obtained by NMR spectroscopy for a 1 MDa proteasome complex. The method relies on immobilization of a soluble protein complex by magic-angle spinning. Deuteration and proton detection of exchangeable sites and paramagnetic relaxation enhancement enables exploration of structural and dynamic properties of supramolecular assemblies at atomic resolution.
Supporting Information
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
| Filename | Description |
|---|---|
| anie_201301215_sm_miscellaneous_information.pdf3.8 MB | miscellaneous_information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
References
- 1K. Wüthrich, NMR of Proteins and Nucleic Acids, Wiley, New York, 1986.
10.1051/epn/19861701011 Google Scholar
- 2A. G. Palmer, J. Williams, A. McDermott, J. Phys. Chem. 1996, 100, 13293–13310.
- 3J. Cavanagh, W. J. Fairbrother, A. G. Palmer III, N. J. Skelton, M. Rance, Protein NMR Spectroscopy, Principles and Practice, 2nd ed., Academic Press, San Diego, 2006.
- 4K. Pervushin, R. Riek, G. Wider, K. Wüthrich, Proc. Natl. Acad. Sci. USA 1997, 94, 12366–12371.
- 5V. Tugarinov, P. M. Hwang, J. E. Ollerenshaw, L. E. Kay, J. Am. Chem. Soc. 2003, 125, 10420–10428.
- 6J. Fiaux, E. B. Bertelsen, A. L. Horwich, K. Wüthrich, Nature 2002, 418, 207–211.
- 7S. Rudiger, S. M. V. Freund, D. B. Veprintsev, A. R. Fersht, Proc. Natl. Acad. Sci. USA 2002, 99, 11085–11090.
- 8I. Gelis, A. M. J. J. Bonvin, D. Keramisanou, M. Koukaki, G. Gouridis, S. Karamanou, A. Economou, C. G. Kalodimos, Cell 2007, 131, 756–769.
- 9R. Sprangers, A. Gribun, P. M. Hwang, W. A. Houry, L. E. Kay, Proc. Natl. Acad. Sci. USA 2005, 102, 16678–16683.
- 10R. Sprangers, L. E. Kay, Nature 2007, 445, 618–622.
- 11R. Sprangers, X. Li, X. Mao, J. L. Rubinstein, A. D. Schimmer, L. E. Kay, Biochemistry 2008, 47, 6727–6734.
- 12E. R. Andrew, A. Bradbury, R. G. Eades, Nature 1958, 182, 1659.
- 13A. Mainz, S. Jehle, B. J. van Rossum, H. Oschkinat, B. Reif, J. Am. Chem. Soc. 2009, 131, 15968–15969.
- 14A. Mainz, B. Bardiaux, F. Kuppler, G. Multhaup, I. C. Felli, R. Pierattelli, B. Reif, J. Biol. Chem. 2012, 287, 1128–1138.
- 15I. Bertini, C. Luchinat, G. Parigi, E. Ravera, B. Reif, P. Turano, Proc. Natl. Acad. Sci. USA 2011, 108, 10396–10399.
- 16E. Ravera, G. Parigi, A. Mainz, T. L. Religa, B. Reif, C. Luchinat, J. Phys. Chem. B 2013, 117, 3548–3553.
- 17W. Baumeister, J. Walz, F. Zühl, E. Seemüller, Cell 1998, 92, 367–380.
- 18J. Löwe, D. Stock, B. Jap, P. Zwickl, W. Baumeister, R. Huber, Science 1995, 268, 533–539.
- 19A. Förster, E. I. Masters, F. G. Whitby, H. Robinson, C. P. Hill, Mol. Cell 2005, 18, 589–599.
- 20V. Chevelkov, K. Rehbein, A. Diehl, B. Reif, Angew. Chem. 2006, 118, 3963–3966;
10.1002/ange.200600328 Google ScholarAngew. Chem. Int. Ed. 2006, 45, 3878–3881.
- 21D. Nietlispach, R. T. Clowes, R. W. Broadhurst, Y. Ito, J. Keeler, M. Kelly, J. Ashurst, H. Oschkinat, P. J. Domaille, E. D. Laue, J. Am. Chem. Soc. 1996, 118, 407–415.
- 22R. Linser, V. Chevelkov, A. Diehl, B. Reif, J. Magn. Reson. 2007, 189, 209–216.
- 23N. P. Wickramasinghe, S. Parthasarathy, C. R. Jones, C. Bhardwaj, F. Long, M. Kotecha, S. Mehboob, L. W.-M. Fung, J. Past, A. Samoson, Y. Ishii, Nat. Methods 2009, 6, 215–218.
- 24R. Linser, J. Biomol. NMR 2012, 52, 151–158.
- 25S. R. Hartmann, E. L. Hahn, Phys. Rev. 1962, 128, 2042–2053.
- 26A. Pines, M. G. Gibby, J. S. Waugh, J. Chem. Phys. 1973, 59, 569–590.
- 27R. Linser, U. Fink, B. Reif, J. Am. Chem. Soc. 2010, 132, 8891–8893.
- 28I. Bertini, F. Engelke, C. Luchinat, G. Parigi, E. Ravera, C. Rosa, P. Turano, Phys. Chem. Chem. Phys. 2012, 14, 439–447.
- 29C. Gardiennet, A. K. Schütz, A. Hunkeler, B. Kunert, L. Terradot, A. Böckmann, B. H. Meier, Angew. Chem. 2012, 124, 7977–7980;
10.1002/ange.201200779 Google ScholarAngew. Chem. Int. Ed. 2012, 51, 7855–7858.
- 30The chemical shift assignment for the α-subunit in 11S-α7β7β7α7-11S has been deposited in the BMRB under accession number 19194.
