Communication

Elucidation of Spore-Photoproduct Formation by Isotope Labeling^†

Dr. Gengjie Lin

Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), 402 North Blackford Street, Indianapolis, IN 46202 (USA), Fax: (+1) 317-274-4701

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Prof. Dr. Lei Li,

Corresponding Author

Prof. Dr. Lei Li

[email protected]

Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), 402 North Blackford Street, Indianapolis, IN 46202 (USA), Fax: (+1) 317-274-4701

Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), 402 North Blackford Street, Indianapolis, IN 46202 (USA), Fax: (+1) 317-274-4701Search for more papers by this author

Dr. Gengjie Lin,

Dr. Gengjie Lin

Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), 402 North Blackford Street, Indianapolis, IN 46202 (USA), Fax: (+1) 317-274-4701

Search for more papers by this author

Prof. Dr. Lei Li,

Corresponding Author

Prof. Dr. Lei Li

[email protected]

Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), 402 North Blackford Street, Indianapolis, IN 46202 (USA), Fax: (+1) 317-274-4701

First published: 23 November 2010

https://doi.org/10.1002/anie.201005228

Citations: 37

^†

We thank Prof. Eric Long at IUPUI for helpful discussions, and the National Institute of Environmental Health Sciences (R00ES017177) as well as the IUPUI startup fund for financial support. The NMR and MS facilities are supported by National Science Foundation MRI grants CHE-0619254 and DBI-0821661, respectively.

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Graphical Abstract

Doing some damage: Spore photoproduct (SP) is the major DNA photodamage product in bacterial endospores. NMR spectroscopic studies of deuterium-labeled TpT dinucleotides have revealed details of the mechanism for SP formation (see scheme). Upon UV irradiation of [D₃]TpT (with a 3′-T CD₃ group), a deuterium atom was transferred exclusively to the 6-H_pro-S position. The migrated atom was a hydrogen atom when [D₄]TpT with a 3′-T CH₃ group was used.

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Volume49, Issue51

December 17, 2010

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Elucidation of Spore-Photoproduct Formation by Isotope Labeling^†

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Elucidation of Spore-Photoproduct Formation by Isotope Labeling^†