A Simple Electrochemical Cytosensor Array for Dynamic Analysis of Carcinoma Cell Surface Glycans†
Wei Cheng
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (China), Fax: (+86) 25-8359-3593
Key Laboratory of Laboratory Medical Diagnostics (Ministry of Education of China), Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China)
Search for more papers by this authorLin Ding
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (China), Fax: (+86) 25-8359-3593
Search for more papers by this authorShijia Ding Prof.
Key Laboratory of Laboratory Medical Diagnostics (Ministry of Education of China), Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China)
Search for more papers by this authorYibing Yin Prof.
Key Laboratory of Laboratory Medical Diagnostics (Ministry of Education of China), Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China)
Search for more papers by this authorHuangxian Ju Prof.
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (China), Fax: (+86) 25-8359-3593
Search for more papers by this authorWei Cheng
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (China), Fax: (+86) 25-8359-3593
Key Laboratory of Laboratory Medical Diagnostics (Ministry of Education of China), Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China)
Search for more papers by this authorLin Ding
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (China), Fax: (+86) 25-8359-3593
Search for more papers by this authorShijia Ding Prof.
Key Laboratory of Laboratory Medical Diagnostics (Ministry of Education of China), Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China)
Search for more papers by this authorYibing Yin Prof.
Key Laboratory of Laboratory Medical Diagnostics (Ministry of Education of China), Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016 (China)
Search for more papers by this authorHuangxian Ju Prof.
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (China), Fax: (+86) 25-8359-3593
Search for more papers by this authorWe gratefully acknowledge the National Science Funds for Creative Research Groups (20821063), Major Research Plan (90713015), Key Program (20535010) and a general project (20875044) from the National Natural Science Foundation of China.
Graphical Abstract
Sugar and spice: The title method for the simultaneous multiplex analysis of intact cell-surface glycans (see picture) shows excellent performance in sensitivity, stability, and practicality. The strategy can be used to analyze the dynamic variation of the cell-surface glycome and to decipher cellular pathophysiological processes.
Supporting Information
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References
- 1K. Ohtsubo, J. D. Marth, Cell 2006, 126, 855.
- 2
- 2aS. Srivastava, J. Proteome Res. 2008, 7, 1799;
- 2bC. J. Bosques, S. Raguram, R. Sasisekharan, Nat. Biotechnol. 2006, 24, 1100;
- 2cJ. E. Turnbull, R. A. Field, Nat. Chem. Biol. 2007, 3, 74;
- 2dM. M. Fuster, J. D. Esko, Nat. Rev. Cancer 2005, 5, 526.
- 3
- 3aA. S. Vercoutter-Edouart, M. C. Slomianny, O. Dekeyzer-Beseme, J. F. Haeuw, J. C. Michalski, Proteomics 2008, 8, 3236;
- 3bC. Ohyama, S. Tsuboi, M. Fukuda, EMBO J. 1999, 18, 1516;
- 3cE. Gorelik, U. Galili, A. Raz, Cancer Metastasis Rev. 2001, 20, 245;
- 3dK. T. Pilobello, D. E. Slawek, L. K. Mahal, Proc. Natl. Acad. Sci. USA 2007, 104, 11534.
- 4
- 4aH. Kaji, H. Saito, Y. Yamauchi, T. Shinkawa, M. Taoka, J. Hirabayashi, K. Kasai, N. Takahashi, T. Isobe, Nat. Biotechnol. 2003, 21, 667;
- 4bR. Naka, S. Kamoda, A. Ishizuka, M. Kinoshita, K. Kakehi, J. Proteome Res. 2006, 5, 88;
- 4cM. D. Sørensen, R. Martins, O. Hindsgaul, Angew. Chem. 2007, 119, 2455;
10.1002/ange.200604936 Google ScholarAngew. Chem. Int. Ed. 2007, 46, 2403.
- 5
- 5aS. Tao, Y. Li, J. Zhou, J. Qian, R. L. Schnaar, Y. Zhang, I. J. Goldstein, H. Zhu, J. P. Schneck, Glycobiology 2008, 18, 761;
- 5bT. Zheng, D. Peelen, L. M. Smith, J. Am. Chem. Soc. 2005, 127, 9982;
- 5cH. Tateno, N. Uchiyama, A. Kuno, A. Togayachi, T. Sato, H. Narimatsu, J. Hirabayashi, Glycobiology 2007, 17, 1138.
- 6
- 6aB. J. Privett, J. H. Shin, M. H. Schoenfisch, Anal. Chem. 2008, 80, 4499;
- 6bC. Spegel, A. Heiskanen, L. H. D. Skjolding, J. Emnéus, Electroanalysis 2008, 20, 680;
- 6cL. Ding, D. Du, X. J. Zhang, H. X. Ju, Curr. Med. Chem. 2008, 15, 3160.
- 7
- 7aW. Cheng, L. Ding, J. P. Lei, S. J. Ding, H. X. Ju, Anal. Chem. 2008, 80, 3867;
- 7bL. Ding, W. Cheng, X. J. Wang, S. J. Ding, H. X. Ju, J. Am. Chem. Soc. 2008, 130, 7224.
- 8
- 8aZ. Lin, Y. Takahashi, T. Murata, M. Takeda, K. Ino, H. Shiku, T. Matsue, Angew. Chem. 2009, 121, 2078; Angew. Chem. Int. Ed. 2009, 48, 2044;
- 8bJ. Wu, F. Yan, X. Q. Zhang, Y. T. Yan, J. H. Tang, H. X. Ju, Clin. Chem. 2008, 54, 1481.
- 9aW. Arap, R. Pasqualini, E. Ruoslahti, Science 1998, 279, 377;
- 9bZ. Dai, A. Kawde, Y. Xiang, J. T. L. Belle, J. Gerlach, V. P. Bhavanandan, L. Joshi, J. Wang, J. Am. Chem. Soc. 2006, 128, 10018;
- 9cK. A. Wearne, H. C. Winter, K. O′Shea, I. J. Goldstein, Glycobiology 2006, 16, 981.
- 10
- 10aA. R. Lizzi, A. M. D′Alessandro, A. Bozzi, B. Cinque, A. Oratore, G. D′Andrea, Mol. Cell. Biochem. 2007, 300, 29;
- 10bR. A. Steet, P. Melançon, R. D. Kuchta, J. Biol. Chem. 2000, 275, 26812.
- 11
- 11aN. Belhacène, L. Maulon, S. Guérin, J. E. Ricci, B. Mari, Y. Colin, J. P. Cartron, P. Auberger, FASEB J. 1998, 12, 531;
- 11bO. Witt, K. Sand, A. Pekrun, Blood 2000, 95, 2391.
- 12aH. Sasaki, B. Bothner, A. Dell, M. Fukuda, J. Biol. Chem. 1987, 262, 12059;
- 12bC. Gahmberg, M. Ekblom, L. C. Andersson, Proc. Natl. Acad. Sci. USA 1984, 81, 6752.
