Volume 46, Issue 32 pp. 6111-6114

Communication

Polymer-Based Elemental Tags for Sensitive Bioassays^†

Xudong Lou,

Xudong Lou

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

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Guohua Zhang,

Guohua Zhang

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

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Isaac Herrera,

Isaac Herrera

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

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Robert Kinach,

Robert Kinach

Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Room 407, Toronto, ON M5S 3G9, Canada, Fax: (+1) 416-978-4317

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Olga Ornatsky Dr.,

Olga Ornatsky Dr.

Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Room 407, Toronto, ON M5S 3G9, Canada, Fax: (+1) 416-978-4317

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Vladimir Baranov Dr.,

Vladimir Baranov Dr.

[email protected]

Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Room 407, Toronto, ON M5S 3G9, Canada, Fax: (+1) 416-978-4317

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Mark Nitz Prof.,

Mark Nitz Prof.

[email protected]

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

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Mitchell A. Winnik Prof.,

Mitchell A. Winnik Prof.

[email protected]

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

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Xudong Lou,

Xudong Lou

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

Search for more papers by this author

Guohua Zhang,

Guohua Zhang

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

Search for more papers by this author

Isaac Herrera,

Isaac Herrera

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

Search for more papers by this author

Robert Kinach,

Robert Kinach

Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Room 407, Toronto, ON M5S 3G9, Canada, Fax: (+1) 416-978-4317

Search for more papers by this author

Olga Ornatsky Dr.,

Olga Ornatsky Dr.

Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Room 407, Toronto, ON M5S 3G9, Canada, Fax: (+1) 416-978-4317

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Vladimir Baranov Dr.,

Vladimir Baranov Dr.

[email protected]

Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Room 407, Toronto, ON M5S 3G9, Canada, Fax: (+1) 416-978-4317

Search for more papers by this author

Mark Nitz Prof.,

Mark Nitz Prof.

[email protected]

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

Search for more papers by this author

Mitchell A. Winnik Prof.,

Mitchell A. Winnik Prof.

[email protected]

Department of Chemistry, University of Toronto, 80 St. George St., Toronto ON M5S 3H6, Canada, Fax: (+1) 416-978-0541

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First published: 03 August 2007

https://doi.org/10.1002/anie.200700796

Citations: 253

^†

This project was funded in part by Genome Canada through the Ontario Genomics Institute and Ontario Cancer Research Network and NIH grant #GM076127-01A1

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Graphical Abstract

It's elemental: A water-soluble polymer bearing multiple metal-chelating ligands has been used as a tag for bioassays with inductively coupled plasma mass spectrometry. The tag was covalently conjugated to antibodies, and the polymer–antibody constructs were loaded with lanthanide ions (Ln³⁺) and used for the simultaneous assay of five orthogonally labeled antibodies against cell surface antigens that differ in abundance by more than two orders of magnitude.

Supporting Information

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Volume46, Issue32

August 13, 2007

Pages 6111-6114

Polymer-Based Elemental Tags for Sensitive Bioassays^†

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Polymer-Based Elemental Tags for Sensitive Bioassays^†