Volume 46, Issue 12 pp. 2023-2027

Communication

siRNA Delivery into Human T Cells and Primary Cells with Carbon-Nanotube Transporters^†

Zhuang Liu,

Zhuang Liu

Department of Chemistry, Stanford University, Stanford, CA 94305, USA, Fax: (+1) 650-725-0259

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Mark Winters Dr.,

Mark Winters Dr.

Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA 94305, USA

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Mark Holodniy Dr.,

Mark Holodniy Dr.

Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA 94305, USA

Palo Alto Health Care System, Stanford, CA 94305, USA

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Hongjie Dai Prof.,

Hongjie Dai Prof.

[email protected]

Department of Chemistry, Stanford University, Stanford, CA 94305, USA, Fax: (+1) 650-725-0259

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Zhuang Liu,

Zhuang Liu

Department of Chemistry, Stanford University, Stanford, CA 94305, USA, Fax: (+1) 650-725-0259

Search for more papers by this author

Mark Winters Dr.,

Mark Winters Dr.

Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA 94305, USA

Search for more papers by this author

Mark Holodniy Dr.,

Mark Holodniy Dr.

Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA 94305, USA

Palo Alto Health Care System, Stanford, CA 94305, USA

Search for more papers by this author

Hongjie Dai Prof.,

Hongjie Dai Prof.

[email protected]

Department of Chemistry, Stanford University, Stanford, CA 94305, USA, Fax: (+1) 650-725-0259

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First published: 02 March 2007

https://doi.org/10.1002/anie.200604295

Citations: 584

^†

We thank the NIH AIDS Reagent Program for providing the three human T cell lines and two MAGI cell lines. This research was supported in part by the Ludwig Translational Research Program at Stanford University and a grant from the NIH/NCI Center for Cancer Nanotechnology Excellence (CCNE). siRNA=short interfering RNA.

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Graphical Abstract

Special delivery: Functionalized single-walled nanotubes (SWNT) can be used as molecular transporters to shuttle short interfering RNA (siRNA) into human T cells and primary cells and silence the expression of HIV-specific cell-surface receptors and coreceptors (see picture; scale bars 40 μm). This silencing effect, known to block HIV viral entry and reduce infection, is superior to that observed with conventional liposome-based nonviral delivery agents.

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Volume46, Issue12

March 12, 2007

Pages 2023-2027

siRNA Delivery into Human T Cells and Primary Cells with Carbon-Nanotube Transporters^†

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siRNA Delivery into Human T Cells and Primary Cells with Carbon-Nanotube Transporters†

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siRNA Delivery into Human T Cells and Primary Cells with Carbon-Nanotube Transporters^†