In Vitro and Intracellular Production of Peptide-Encapsulated Fluorescent Silver Nanoclusters†
The authors gratefully acknowledge financial support from NSF BES-0323453, Invitrogen Corp., NIH R01M68732, and NIH P20072021, and thank Prof. D. F. Doyle for use of his cell-culture facility, and Prof. Y.-L. Tzeng for the gift of the peptides.
Graphical Abstract
Every nucleolus has a silver lining: Formation of silver nanoclusters by fluorescence photoactivation was used for the staining of cells at low silver nitrate concentrations and ambient temperature. The picture shows picosecond lifetime images of peptide-encapsulated silver nanoclusters within NIH 3T3 cells.