Design and Synthesis of Photochemically Controllable Caspase-3†
Masayuki Endo Dr.
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan, Fax: (+81) 6-6879-8499
Search for more papers by this authorKoji Nakayama
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan, Fax: (+81) 6-6879-8499
Search for more papers by this authorYuka Kaida
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan, Fax: (+81) 6-6879-8499
Search for more papers by this authorTetsuro Majima Dr.
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan, Fax: (+81) 6-6879-8499
Search for more papers by this authorMasayuki Endo Dr.
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan, Fax: (+81) 6-6879-8499
Search for more papers by this authorKoji Nakayama
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan, Fax: (+81) 6-6879-8499
Search for more papers by this authorYuka Kaida
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan, Fax: (+81) 6-6879-8499
Search for more papers by this authorTetsuro Majima Dr.
Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567–0047, Japan, Fax: (+81) 6-6879-8499
Search for more papers by this authorWe thank Dr. Yoshihide Tsujimoto (Osaka University Medical School) for providing cDNA of human caspase-3. This work was supported by a Grant-in-Aid for Scientific Research on Priority Area (417), 21st Century COE Research, and from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of the Japanese Government.
Graphical Abstract
The selective cleavage achieved by activated caspase-8 has been mimicked by using a photofunctionalized caspase-3 having 2-nitrophenylglycine (Npg) at a specific position of the peptide chain (see picture). The study shows that the activity of caspase-3 has been clearly expressed by photoirradiation and that autocleavage of caspase-3 has been suppressed by the site-selective incorporation of the Npg residue.
Supporting Information
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