Side-Chain Chemistry Governs Hierarchical Order of Charge-Complementary β-sheet Peptide Coassemblies
Dr. Renjie Liu
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
These authors contributed equally to this work.
Search for more papers by this authorXin Dong
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC-27695 USA
These authors contributed equally to this work.
Search for more papers by this authorDr. Dillon T. Seroski
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
These authors contributed equally to this work.
Search for more papers by this authorDr. Bethsymarie Soto Morales
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
Search for more papers by this authorDr. Kong M. Wong
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA-30332 USA
Search for more papers by this authorAlicia S. Robang
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA-30332 USA
Search for more papers by this authorLucas Melgar
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
Search for more papers by this authorProf. Thomas E. Angelini
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL-32611 USA
Search for more papers by this authorProf. Anant K. Paravastu
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA-30332 USA
Search for more papers by this authorProf. Carol K. Hall
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC-27695 USA
Search for more papers by this authorCorresponding Author
Prof. Gregory A. Hudalla
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
Search for more papers by this authorDr. Renjie Liu
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
These authors contributed equally to this work.
Search for more papers by this authorXin Dong
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC-27695 USA
These authors contributed equally to this work.
Search for more papers by this authorDr. Dillon T. Seroski
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
These authors contributed equally to this work.
Search for more papers by this authorDr. Bethsymarie Soto Morales
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
Search for more papers by this authorDr. Kong M. Wong
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA-30332 USA
Search for more papers by this authorAlicia S. Robang
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA-30332 USA
Search for more papers by this authorLucas Melgar
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
Search for more papers by this authorProf. Thomas E. Angelini
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL-32611 USA
Search for more papers by this authorProf. Anant K. Paravastu
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA-30332 USA
Search for more papers by this authorProf. Carol K. Hall
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC-27695 USA
Search for more papers by this authorCorresponding Author
Prof. Gregory A. Hudalla
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL-32611 USA
Search for more papers by this authorAbstract
Self-assembly of proteinaceous biomolecules into functional materials with ordered structures that span length scales is common in nature yet remains a challenge with designer peptides under ambient conditions. This report demonstrates how charged side-chain chemistry affects the hierarchical co-assembly of a family of charge-complementary β-sheet-forming peptide pairs known as CATCH(X+/Y−) at physiologic pH and ionic strength in water. In a concentration-dependent manner, the CATCH(6K+) (Ac-KQKFKFKFKQK-Am) and CATCH(6D−) (Ac-DQDFDFDFDQD-Am) pair formed either β-sheet-rich microspheres or β-sheet-rich gels with a micron-scale plate-like morphology, which were not observed with other CATCH(X+/Y−) pairs. This hierarchical order was disrupted by replacing D with E, which increased fibril twisting. Replacing K with R, or mutating the N- and C-terminal amino acids in CATCH(6K+) and CATCH(6D−) to Qs, increased observed co-assembly kinetics, which also disrupted hierarchical order. Due to the ambient assembly conditions, active CATCH(6K+)-green fluorescent protein fusions could be incorporated into the β-sheet plates and microspheres formed by the CATCH(6K+/6D−) pair, demonstrating the potential to endow functionality.
Conflict of interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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