Precise Epitope Organization with Self-adjuvant Framework Nucleic Acid for Efficient COVID-19 Peptide Vaccine Construction
Dr. Fengyun Shen
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China
Contribution: Conceptualization (lead), Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Writing - original draft (lead)
Search for more papers by this authorZijian Xiong
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
Contribution: Data curation (supporting), Investigation (equal)
Search for more papers by this authorDr. Yumin Wu
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
Contribution: Investigation (supporting), Methodology (supporting)
Search for more papers by this authorProf. Dr. Rui Peng
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
Contribution: Supervision (supporting)
Search for more papers by this authorYue Wang
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China
Contribution: Methodology (supporting)
Search for more papers by this authorCorresponding Author
Dr. Lele Sun
School of Life Sciences, Shanghai University, 200444 Shanghai, China
Contribution: Conceptualization (lead), Funding acquisition (lead), Investigation (lead), Methodology (lead), Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorProf. Dr. Chunhai Fan
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China
Contribution: Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorCorresponding Author
Prof. Dr. Zhuang Liu
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
Contribution: Conceptualization (equal), Funding acquisition (lead), Supervision (equal), Writing - review & editing (lead)
Search for more papers by this authorDr. Fengyun Shen
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China
Contribution: Conceptualization (lead), Data curation (lead), Formal analysis (lead), Investigation (lead), Methodology (lead), Writing - original draft (lead)
Search for more papers by this authorZijian Xiong
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
Contribution: Data curation (supporting), Investigation (equal)
Search for more papers by this authorDr. Yumin Wu
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
Contribution: Investigation (supporting), Methodology (supporting)
Search for more papers by this authorProf. Dr. Rui Peng
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
Contribution: Supervision (supporting)
Search for more papers by this authorYue Wang
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China
Contribution: Methodology (supporting)
Search for more papers by this authorCorresponding Author
Dr. Lele Sun
School of Life Sciences, Shanghai University, 200444 Shanghai, China
Contribution: Conceptualization (lead), Funding acquisition (lead), Investigation (lead), Methodology (lead), Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorProf. Dr. Chunhai Fan
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, 201240 Shanghai, China
Contribution: Supervision (equal), Writing - review & editing (equal)
Search for more papers by this authorCorresponding Author
Prof. Dr. Zhuang Liu
Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Lab Carbon Based Functional Materials and Devices, Soochow University, 215123 Suzhou, Jiangsu, China
Contribution: Conceptualization (equal), Funding acquisition (lead), Supervision (equal), Writing - review & editing (lead)
Search for more papers by this authorAbstract
Peptide vaccines have advantages in easy fabrication and high safety, but their effectiveness is hampered by the poor immunogenicity of the epitopes themselves. Herein, we constructed a series of framework nucleic acids (FNAs) with regulated rigidity and size to precisely organize epitopes in order to reveal the influence of epitope spacing and carrier rigidity on the efficiency of peptide vaccines. We found that assembling epitopes on rigid tetrahedral FNAs (tFNAs) with the appropriate size could efficiently enhance their immunogenicity. Further, by integrating epitopes from SARS-CoV-2 on preferred tFNAs, we constructed a COVID-19 peptide vaccine which could induce high titers of IgG against the receptor binding domain (RBD) of SARS-CoV-2 spike protein and increase the ratio of memory B and T cells in mice. Considering the good biocompatibility of tFNAs, our research provides a new idea for developing efficient peptide vaccines against viruses and possibly other diseases.
Conflict of interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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