Optical Cell Tagging for Spatially Resolved Single-Cell RNA Sequencing
Qi Tang
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
These authors contributed equally to this work.
Search for more papers by this authorLu Liu
Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry and Molecular Engineering, Beijing Advanced Innovation Center for Genomics (ICG), Peking-Tsinghua Center for Life Science, and Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871 China
These authors contributed equally to this work.
Search for more papers by this authorYilan Guo
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorXu Zhang
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorShaoran Zhang
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorYan Jia
Renal Division, Peking University First Hospital, Beijing, 100034 China
Institute of Nephrology, Key Laboratory of CKD Prevention and Treatment of Ministry of Education of China, Peking University, Beijing, 100871 China
Research Units of Diagnosis and Treatment of Immune-mediated Kidney Diseases, Chinese Academy of Medical Sciences, Beijing, 100730 China
Search for more papers by this authorYifei Du
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorBo Cheng
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorCorresponding Author
Prof. Li Yang
Renal Division, Peking University First Hospital, Beijing, 100034 China
Institute of Nephrology, Key Laboratory of CKD Prevention and Treatment of Ministry of Education of China, Peking University, Beijing, 100871 China
Research Units of Diagnosis and Treatment of Immune-mediated Kidney Diseases, Chinese Academy of Medical Sciences, Beijing, 100730 China
Search for more papers by this authorCorresponding Author
Prof. Yanyi Huang
Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry and Molecular Engineering, Beijing Advanced Innovation Center for Genomics (ICG), Peking-Tsinghua Center for Life Science, and Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871 China
Search for more papers by this authorCorresponding Author
Prof. Xing Chen
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorQi Tang
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
These authors contributed equally to this work.
Search for more papers by this authorLu Liu
Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry and Molecular Engineering, Beijing Advanced Innovation Center for Genomics (ICG), Peking-Tsinghua Center for Life Science, and Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871 China
These authors contributed equally to this work.
Search for more papers by this authorYilan Guo
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorXu Zhang
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorShaoran Zhang
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorYan Jia
Renal Division, Peking University First Hospital, Beijing, 100034 China
Institute of Nephrology, Key Laboratory of CKD Prevention and Treatment of Ministry of Education of China, Peking University, Beijing, 100871 China
Research Units of Diagnosis and Treatment of Immune-mediated Kidney Diseases, Chinese Academy of Medical Sciences, Beijing, 100730 China
Search for more papers by this authorYifei Du
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorBo Cheng
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorCorresponding Author
Prof. Li Yang
Renal Division, Peking University First Hospital, Beijing, 100034 China
Institute of Nephrology, Key Laboratory of CKD Prevention and Treatment of Ministry of Education of China, Peking University, Beijing, 100871 China
Research Units of Diagnosis and Treatment of Immune-mediated Kidney Diseases, Chinese Academy of Medical Sciences, Beijing, 100730 China
Search for more papers by this authorCorresponding Author
Prof. Yanyi Huang
Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry and Molecular Engineering, Beijing Advanced Innovation Center for Genomics (ICG), Peking-Tsinghua Center for Life Science, and Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871 China
Search for more papers by this authorCorresponding Author
Prof. Xing Chen
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871 China
Search for more papers by this authorAbstract
Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for profiling gene expression of distinct cell populations at the single-cell level. However, the information of the positions of cells within the multicellular samples is missing in scRNA-seq datasets. To overcome this limitation, we herein develop OpTAG (optical cell tagging) as a new chemical platform for attaching functional tags onto cell surfaces in a spatially resolved manner. With OpTAG, we establish OpTAG-seq, which enables spatially resolved scRNA-seq. We apply OpTAG-seq to investigate the spatially defined transcriptional program in migrating cancer cells and identified a list of genes that are potential regulators for cancer cell migration and invasion. OpTAG-seq provides a convenient method for mapping cellular heterogeneity with spatial information within multicellular biological systems.
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