Calprotectin in chronic rhinosinusitis eosinophil extracellular traps
Sang Hyeon Ahn MD, PhD
Department of Otorhinolaryngology, Bundang Jesaeng General Hospital, Daejin Medical Center, Seongnam, South Korea
Department of Medicine, The Graduate School of Yonsei University, Seoul, South Korea
Search for more papers by this authorKyu Ha Shin MD
Department of Otorhinolaryngology, Bundang Jesaeng General Hospital, Daejin Medical Center, Seongnam, South Korea
Search for more papers by this authorJun Taek Oh MD
Department of Otorhinolaryngology, Bundang Jesaeng General Hospital, Daejin Medical Center, Seongnam, South Korea
Search for more papers by this authorSang Chul Park MD, PhD
Department of otorhinolaryngology, Hallym University Kangnam Sacred Heart Hospital, Seoul, South Korea
Search for more papers by this authorMin-Seok Rha MD, PhD
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea
Search for more papers by this authorSoo In Kim BS
Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea
Korea Mouse Sensory Phenotyping Center, Seoul, South Korea
Search for more papers by this authorHyung-Ju Cho MD, PhD
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
Korea Mouse Sensory Phenotyping Center, Seoul, South Korea
The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, South Korea
Search for more papers by this authorJoo-Heon Yoon MD, PhD
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
Korea Mouse Sensory Phenotyping Center, Seoul, South Korea
The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, South Korea
Global Research Laboratory for Allergic Airway Disease, Yonsei University College of Medicine, Seoul, South Korea
Search for more papers by this authorCorresponding Author
Chang-Hoon Kim MD, PhD
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
Korea Mouse Sensory Phenotyping Center, Seoul, South Korea
The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, South Korea
Medical Research Center, Yonsei University College of Medicine, Seoul, South Korea
Correspondence
Chang-Hoon Kim, MD, PhD, Department of Otorhinolaryngology, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemoon-gu, 03722 Seoul, South Korea.
Email: [email protected]
Search for more papers by this authorSang Hyeon Ahn MD, PhD
Department of Otorhinolaryngology, Bundang Jesaeng General Hospital, Daejin Medical Center, Seongnam, South Korea
Department of Medicine, The Graduate School of Yonsei University, Seoul, South Korea
Search for more papers by this authorKyu Ha Shin MD
Department of Otorhinolaryngology, Bundang Jesaeng General Hospital, Daejin Medical Center, Seongnam, South Korea
Search for more papers by this authorJun Taek Oh MD
Department of Otorhinolaryngology, Bundang Jesaeng General Hospital, Daejin Medical Center, Seongnam, South Korea
Search for more papers by this authorSang Chul Park MD, PhD
Department of otorhinolaryngology, Hallym University Kangnam Sacred Heart Hospital, Seoul, South Korea
Search for more papers by this authorMin-Seok Rha MD, PhD
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea
Search for more papers by this authorSoo In Kim BS
Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea
Korea Mouse Sensory Phenotyping Center, Seoul, South Korea
Search for more papers by this authorHyung-Ju Cho MD, PhD
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
Korea Mouse Sensory Phenotyping Center, Seoul, South Korea
The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, South Korea
Search for more papers by this authorJoo-Heon Yoon MD, PhD
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
Korea Mouse Sensory Phenotyping Center, Seoul, South Korea
The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, South Korea
Global Research Laboratory for Allergic Airway Disease, Yonsei University College of Medicine, Seoul, South Korea
Search for more papers by this authorCorresponding Author
Chang-Hoon Kim MD, PhD
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
Korea Mouse Sensory Phenotyping Center, Seoul, South Korea
The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, South Korea
Medical Research Center, Yonsei University College of Medicine, Seoul, South Korea
Correspondence
Chang-Hoon Kim, MD, PhD, Department of Otorhinolaryngology, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemoon-gu, 03722 Seoul, South Korea.
Email: [email protected]
Search for more papers by this authorAbstract
Background
Calprotectin is an antimicrobial peptide primarily secreted by neutrophils. Furthermore, calprotectin secretion increases in patients with chronic rhinosinusitis (CRS) with polyps (CRSwNP) and positively correlates with neutrophil markers. However, CRSwNP is known to be associated with type 2 inflammation related to tissue eosinophilia. Therefore, the authors investigated calprotectin expression in eosinophils and eosinophil extracellular traps (EETs) and explored the associations between tissue calprotectin and the clinical findings of patients with CRS.
Methods
A total of 63 patients participated, and patients diagnosed with CRS were classified based on the Japanese Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis (JESREC) score. The authors performed hematoxylin and eosin staining, immunohistochemistry, immunofluorescence with calprotectin, myeloperoxidase (MPO), major basic protein (MBP), and citrullinated histone H3 with the participant's tissues. Finally, correlations between calprotectin and the clinical data were examined.
Results
Calprotectin-positive cells are co-localized not only in MPO-positive cells but also in MBP-positive cells in human tissues. Calprotectin was also involved in EETs and neutrophil extracellular traps. The number of calprotectin-positive cells in the tissue was positively correlated with the number of tissue and blood eosinophils. In addition, calprotectin in the tissue is associated with the olfactory function, Lund–Mackay computed tomography score, and JESREC score.
Conclusions
Calprotectin, known to be secreted by neutrophils, in CRS was also expressed in eosinophils. In addition, calprotectin, which functions as an antimicrobial peptide, may play an important role in the innate immune response based on its EET involvement. Therefore, calprotectin expression could reflect as a disease severity biomarker for CRS.
CONFLICT OF INTEREST STATEMENT
The authors declare no conflicts of interest.
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