International Forum of Allergy & Rhinology

ORIGINAL ARTICLE

Sox2 regulates globose basal cell regeneration in the olfactory epithelium

Zhexuan Li MD

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China

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Ming Wei MD,

Ming Wei MD

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China

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Wenjuan Shen PhD,

Wenjuan Shen PhD

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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Heather Kulaga MS,

Heather Kulaga MS

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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Mengfei Chen PhD,

Mengfei Chen PhD

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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Andrew P. Lane MD,

Corresponding Author

Andrew P. Lane MD

[email protected]

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Correspondence

Andrew P. Lane, MD, Department of Otolaryngology–Head and Neck Surgery, Johns Hopkins Outpatient Center, 6th floor,601 N Caroline Street, Baltimore, MD 21287-0910, USA.

Email: [email protected]

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Zhexuan Li MD,

Zhexuan Li MD

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China

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Ming Wei MD,

Ming Wei MD

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China

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Wenjuan Shen PhD,

Wenjuan Shen PhD

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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Heather Kulaga MS,

Heather Kulaga MS

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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Mengfei Chen PhD,

Mengfei Chen PhD

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

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Andrew P. Lane MD,

Corresponding Author

Andrew P. Lane MD

[email protected]

Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

Correspondence

Andrew P. Lane, MD, Department of Otolaryngology–Head and Neck Surgery, Johns Hopkins Outpatient Center, 6th floor,601 N Caroline Street, Baltimore, MD 21287-0910, USA.

Email: [email protected]

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First published: 26 September 2021

https://doi.org/10.1002/alr.22890

Citations: 4

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Abstract

Background

In the olfactory epithelium, mitotically active globose basal cells (GBCs) continuously replenish olfactory sensory neurons (OSNs) lost throughout life. Although an essential role of the transcription factor Sox2 in expanding olfactory progenitors/stem cells has been shown, its precise role in olfactory GBCs remain incompletely understood.

Methods

We characterized the Sox2 expression in olfactory GBCs in normal conditions and in a lesion-regeneration model using a Lgr5EGFP-IRES-creERT2 strain. During GBC-mediated regeneration, genetic deletion of sox2 and lineage tracing experiments were performed to examine the function of Sox2 in the progeny of Lgr5-EGFP⁺ GBCs.

Results

Over 95% of Lgr5-EGFP⁺ GBCs express Sox2 in normal or regeneration conditions. Loss of Sox2 dramatically reduces the cell number in each lineage traced cluster. In the progeny of Lgr5-EGFP⁺ GBCs, loss of Sox2 significantly decreased the portion of OMP⁺ OSNs. However, the generation of sustentacular cells was unchanged.

Conclusions

Our observations support an essential role of Sox2 in adult olfactory regeneration, likely acting on neuronal-lineage GBCs.

CONFLICTS OF INTEREST

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could role as a potential conflict of interest.

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Volume12, Issue3

March 2022

Pages 286-292

Sox2 regulates globose basal cell regeneration in the olfactory epithelium

Abstract

Background

Methods

Results

Conclusions

CONFLICTS OF INTEREST

REFERENCES

Citing Literature

References

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Sox2 regulates globose basal cell regeneration in the olfactory epithelium

Abstract

Background

Methods

Results

Conclusions

CONFLICTS OF INTEREST

REFERENCES

Citing Literature

References

Related

Information