Chapter 6

IR and Raman Spectroscopy for Pathogen Detection

Ute Münchberg

Ute Münchberg

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany

Jena School for Microbial Communication, Friedrich Schiller University Jena, Neugasse 23,07743, Jena, Germany

Search for more papers by this author
Sandra Kloß

Sandra Kloß

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany

Search for more papers by this author
Dragana Kusić

Dragana Kusić

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany

Search for more papers by this author
Susann Meisel

Susann Meisel

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany

Search for more papers by this author
Ralf Heinke

Ralf Heinke

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany

Search for more papers by this author
Stephan Stöckel

Stephan Stöckel

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany

Search for more papers by this author
Petra Rösch

Petra Rösch

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany

Search for more papers by this author
Jürgen Popp

Jürgen Popp

Prof. Dr.

Friedrich-Schiller University Jena and Abbe Center of Photonics, Institute of Physical Chemistry, Helmholtzweg 4, 07743 Jena, Germany

Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany

Search for more papers by this author
First published: 27 February 2015

Summary

This chapter presents an overview over the different vibrational spectroscopic approaches in the field of bacterial identification. It focuses on the infrared (IR) and Raman spectroscopy of bulk samples and discusses some approaches for single cell analysis. The investigation of microorganisms with spectroscopic techniques is frequently carried out on bulk materials. To obtain this, the samples have to be cultivated. This offers the opportunity to standardize the growth conditions in order to generate uniform samples. The single bacterial cells have to be brought on a Raman-compatible substrate such as fused silica, calcium fluoride, or nickel to measure the Raman spectra. Latest research frequently aims to transfer the approach of Raman spectroscopic identification of single cells to real-world applications. These applications can be very diverse, for example, ranging from water analysis to pathogen identification, biothreat detection, and also food safety issues.

The full text of this article hosted at iucr.org is unavailable due to technical difficulties.