IR and Raman Spectroscopy for Pathogen Detection
Ute Münchberg
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Jena School for Microbial Communication, Friedrich Schiller University Jena, Neugasse 23,07743, Jena, Germany
Search for more papers by this authorSandra Kloß
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorDragana Kusić
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorSusann Meisel
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorRalf Heinke
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorStephan Stöckel
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorPetra Rösch
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorJürgen Popp
Prof. Dr.
Friedrich-Schiller University Jena and Abbe Center of Photonics, Institute of Physical Chemistry, Helmholtzweg 4, 07743 Jena, Germany
Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany
Search for more papers by this authorUte Münchberg
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Jena School for Microbial Communication, Friedrich Schiller University Jena, Neugasse 23,07743, Jena, Germany
Search for more papers by this authorSandra Kloß
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorDragana Kusić
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorSusann Meisel
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorRalf Heinke
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorStephan Stöckel
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorPetra Rösch
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
Search for more papers by this authorJürgen Popp
Prof. Dr.
Friedrich-Schiller University Jena and Abbe Center of Photonics, Institute of Physical Chemistry, Helmholtzweg 4, 07743 Jena, Germany
Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany
Search for more papers by this authorJürgen Popp
Prof. Dr.
Friedrich-Schiller University Jena and Abbe Center of Photonics, Institute of Physical Chemistry, Helmholtzweg 4, 07743 Jena, Germany
Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany
Search for more papers by this authorMichael Bauer
Prof. Dr.
Jena University Hospital, Department of Anesthesiology and Intensive Care Medicine, Germany
Center for Sepsis Control and Care (CSCC), Erlanger Allee 101, 07747 Jena, Germany
Search for more papers by this authorSummary
This chapter presents an overview over the different vibrational spectroscopic approaches in the field of bacterial identification. It focuses on the infrared (IR) and Raman spectroscopy of bulk samples and discusses some approaches for single cell analysis. The investigation of microorganisms with spectroscopic techniques is frequently carried out on bulk materials. To obtain this, the samples have to be cultivated. This offers the opportunity to standardize the growth conditions in order to generate uniform samples. The single bacterial cells have to be brought on a Raman-compatible substrate such as fused silica, calcium fluoride, or nickel to measure the Raman spectra. Latest research frequently aims to transfer the approach of Raman spectroscopic identification of single cells to real-world applications. These applications can be very diverse, for example, ranging from water analysis to pathogen identification, biothreat detection, and also food safety issues.
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