Dynamics of Membrane Receptors: Single-molecule Tracking of Quantum Dot Liganded Epidermal Growth Factor
Guy M. Hagen
Department of Cell Biology, Charles University Medical School, Albertor 4, 12800 Prague, Czech Republic
Search for more papers by this authorKeith A. Lidke
University of New Mexico, Department of Physics, Albuquerque, NM 87131, USA
Search for more papers by this authorBernd Rieger
Delft University of Technology, Department of Imaging Science & Technology, Quantitative Imaging Group, Lorentzweg 1, 2628 CJ Delft, The Netherlands
Search for more papers by this authorDiane S. Lidke
University of New Mexico, Department of Pathology, Albuquerque, NM 87131, USA
Search for more papers by this authorWouter Caarls
Max Planck Institute for Biophysical Chemistry, Laboratory of Cellular Dynamics, Am Faßberg 11, 37077 Göttingen, Germany
Search for more papers by this authorDonna J. Arndt-Jovin
Max Planck Institute for Biophysical Chemistry, Laboratory of Cellular Dynamics, Am Faßberg 11, 37077 Göttingen, Germany
Search for more papers by this authorThomas M. Jovin
Max Planck Institute for Biophysical Chemistry, Laboratory of Cellular Dynamics, Am Faßberg 11, 37077 Göttingen, Germany
Search for more papers by this authorGuy M. Hagen
Department of Cell Biology, Charles University Medical School, Albertor 4, 12800 Prague, Czech Republic
Search for more papers by this authorKeith A. Lidke
University of New Mexico, Department of Physics, Albuquerque, NM 87131, USA
Search for more papers by this authorBernd Rieger
Delft University of Technology, Department of Imaging Science & Technology, Quantitative Imaging Group, Lorentzweg 1, 2628 CJ Delft, The Netherlands
Search for more papers by this authorDiane S. Lidke
University of New Mexico, Department of Pathology, Albuquerque, NM 87131, USA
Search for more papers by this authorWouter Caarls
Max Planck Institute for Biophysical Chemistry, Laboratory of Cellular Dynamics, Am Faßberg 11, 37077 Göttingen, Germany
Search for more papers by this authorDonna J. Arndt-Jovin
Max Planck Institute for Biophysical Chemistry, Laboratory of Cellular Dynamics, Am Faßberg 11, 37077 Göttingen, Germany
Search for more papers by this authorThomas M. Jovin
Max Planck Institute for Biophysical Chemistry, Laboratory of Cellular Dynamics, Am Faßberg 11, 37077 Göttingen, Germany
Search for more papers by this authorProf. Dr. Toshio Yanagida
Osaka University, Graduate School of Frontier Bioscience and Graduate School of Medicine, 1-3 Yamada-oka, Suita, Osaka 562-0871, Japan
Search for more papers by this authorDr. Yoshiharu Ishii
CREST, Soft nano-machine Project JST, 1-3 Yamada-oka, Suita, Osaka 562-0871, Japan
Search for more papers by this authorSummary
This chapter contains sections titled:
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Introduction
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Single QD Imaging
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Retrograde Transport of Activated EGFR Dimers
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Single QD–EGF–EGFR Tracking
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Programmable Array Microscopy
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Concluding Remarks
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Appendix A: Materials and Methods
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Appendix B: Software and Image Processing
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References
References
- Yarden, Y. and Sliwkowski (2001) Untangling the ErbB signaling network. Nat. Rev. Mol. Cell Biol., 2, 127–137.
- Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H.E., Jares-Erijman, E.A. and Jovin, T.M. (2004) Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction. Nat. Biotech., 22, 198.
- Lidke, D.S., Lidke, K.A., Rieger, B., Jovin, T.M. and Arndt-Jovin, D.J. (2005) Reaching out for signals: filopodia sense EGF and respond by directed retrograde transport of activated receptors. J. Cell Biol., 170, 619–626.
- Grecco, H.E., Lidke, K.A., Heintzmann, R., Lidke, D.S., Spagnuolo, C., Martinez, O.E., Jares-Erijman, E.A. and Jovin, T.M. (2004) Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells. Microsc. Res. Tech., 65, 169–179.
- Jares-Erijman, E.A. and Jovin, T.M. (2003) FRET imaging. Nat. Biotech., 21, 1387.
- Coates, C.G., Denvir, D.J., McHale, N.G., Thornbury, K.D. and Hollywood, M.A. (2004) Optimizing low-light microscopy with back-illuminated electron multiplying charge-coupled device: enhanced sensitivity, speed, and resolution. J. Biomed. Opt., 9, 1244–1252.
- Miskoski, S., Giordano, L., Etchehon, M.H., Menendez, G., Lidke, K.A., Hagen, G.M., Jovin, T.M. and Jares-Erijman, E.A. (2006) Spectroscopic modulation of multifunctionalized quantum dots for use as biological probes and effectors. Proc. SPIE 6096, 60960X.
- Lidke, K.A., Rieger, B., Jovin, T.M. and Heintzmann, R. (2005) Superresolution by localization of quantum dots using blinking statistics. Opt. Express, 12, 7052–7062.
- Suzuki, K., Ritchie, K., Kajikawa, E., Fujiwara, T. and Kusumi, A. (2005) Rapid hop diffusion of a G-protein-coupled receptor in the plasma membrane as revealed by single-molecule techniques. Biophys. J., 88, 3659–3680.
- Hanley, Q.S., Verveer, P., Gemkow, M., Arndt-Jovin, D.J. and Jovin, T.M. (1999) An optical sectioning programmable array microscope implemented with a digital micromirror device. J. Microsc., 196, 317–331.
- Hanley, Q.S. and Jovin, T.M. (2001) Highly multiplexed optically sectioned spectroscopic imaging in a programmable array microscope. App. Spectrosc., 55, 1115.
- Hanley, Q.S., Lidke, K.A., Heintzmann, R., Arndt-Jovin, D.J. and Jovin, T.M. (2005) Fluorescence lifetime imaging in an optically sectioning programmable array microscope (PAM). Cytometry Part A, 67A, 112–118.
- Heintzmann, R., Hanley, Q.S., Arndt-Jovin, D. and Jovin, T.M. (2001) A dual path programmable array microscope (PAM): simultaneous acquisition of conjugate and non-conjugate images. J. Microsc., 204, 119–135.
- Liang, M., Stehr, R.L. and Krause, A.W., (1997) Confocal pattern period in multiple-aperture confocal imaging systems with coherent illumination. Opt. Lett., 22, 751–753.
- Fukano, T. and Miyawaki, A. (2003) Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples. Appl. Opt., 42, 4119–4124.
- Cha, S., Lin, P.C., Zhu, L., Sun, P.-C. and Fainman, Y. (2000) Nontranslational three-dimensional profilometry by chromatic confocal microscopy with dynamically configurable micromirror scanning. Appl. Opt., 39, 2605–2613.
- Lane, P.M., Dlugan, A.L.P., Richards-Kortum, R. and MacAulay, C.E. (2000) Fiber-optic confocal microscopy using a spatial light modulator. Opt. Lett, 25, 1780–1782.
- Smith, P.J., Taylor, C.M., Shaw, A.J. and McCabe, E.M. (2000) Programmable array microscopy with a ferroelectric liquid-crystal spatial light modulator. Appl. Opt., 39, 2664–2669.
- Neil, M.A.A., Juskaitis, R. and Wilson, T., (1997) Method of obtaining optical sectioning by using structured light in a conventional microscope. Opt. Lett, 22, 1905–1907.
- Harwit, M. and Sloane, N.J.A., (1979) Hadamard Transform Optics, Academic Press, New York.
- Heintzmann, R. and Benedetti, P.A. (2006) High-resolution image reconstruction in fluorescence microscopy with patterned excitation. Appl. Opt., 45, 5037–5045.
- Buck, I., Foley, T., Horn, D., Sugerman, J., Fatahalian, K., Houston, M. and Hanrahan, P. (2004) Brook for GPUs: Stream Computing on Graphics Hardware. ACM Trans. Graph., 23, 777–786.
- Arndt-Jovin, D.J., Lopez-Quintela, M.A., Lidke, D.S., Rodriguez, M.J., Santos, F.M., Lidke, K.A., Hagen, G.M. and Jovin, T.M. (2006) In vivo cell imaging with semiconductor quantum dots and noble metal nanodots. Proc. SPIE 6096 60960P.
- Fulwyler, M.J. Hanley, Q.S., Schnetter, C., Young, I.T., Jares-Erijman, E., Arndt-Jovin, D.J. and Jovin, T.M. (2005) Selective photoreactions in a Programmable Array Microscope (PAM): Photoinitiated polymerization, photodecaging and photochromic conversion. Cytometry, 67A, 68–75.
- Fry, D.W., Kraker, A.J., McMichael, A., Ambroso, L.A., Nelson, J.M., Leopold, W.R., Connors, R.W. and Bridges, A.J., (1994) A specific inhibitor of the epidermal growth factor receptor tyrosine kinase. Science, 265, 1093–1095.
