This investigation was done on 10 healthy subjects which includes both males and females in the age set of 20-30 years (mean: 24± 1.944 years). The examination was performed to survey the impact of graded HUT and HRT on the sympathetic and parasympathetic nervous systems. The tilt angles taken to record the data were 0 ⁰ , 20 ⁰ , 40 ⁰ , 60 ⁰ , 40 ⁰ R, 20 ⁰ R, and 0 ⁰ R. After that, time domain and frequency domain parameters were analysed. On evaluated head-up tilt (0 ⁰ to 60 ⁰ ), the mean heart rate, LF, and LF/HF showed a growing trend and the mean RR, NN50, pNN50, and HF showed a declining trend. On reversal of the tilt (60 ⁰ to 0 ⁰ R), all the parameters showed an inverse trend as that of the trend from 0 ⁰ to 60 ⁰ . These trends clearly depict that graded HUT leads to an escalation in sympathetic activity but a decrease in parasympathetic activity, which is clearly seen at higher tilt angles (20 ⁰ to 60 ⁰ ). When the tilt-table was reversed, both the parasympathetic and sympathetic activity of ANS came back to regular pre-tilt level. The tilt-table test is basic and non-invasive and can be easily utilized clinically for the analysis and affirmation of syncope dysfunction of the ANS.