In vivo measurement of the size of lipid droplets in an intracerebral glioma in the rat
Corresponding Author
Hana Lahrech
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
INSERM U438, Centre Hospitalier Universitaire, Pavillon B, BP 217, F 38043 Grenoble cedex 9, France===Search for more papers by this authorSonia Zoula
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Search for more papers by this authorRégine Farion
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Search for more papers by this authorChantal Rémy
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Search for more papers by this authorMichel Décorps
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Search for more papers by this authorCorresponding Author
Hana Lahrech
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
INSERM U438, Centre Hospitalier Universitaire, Pavillon B, BP 217, F 38043 Grenoble cedex 9, France===Search for more papers by this authorSonia Zoula
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Search for more papers by this authorRégine Farion
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Search for more papers by this authorChantal Rémy
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Search for more papers by this authorMichel Décorps
Unité mixte INSERM–Université Joseph Fourier, Hôpital Albert Michallon, Grenoble, France
Search for more papers by this authorAbstract
Pulsed field gradient NMR was used to measure the root mean square displacement λ of the NMR visible lipid molecules in C6 brain tumors in the rat at different diffusion times. For a distribution of spherical droplets of diameter with volume fraction ξ(Φi), the mean characteristic droplet diameter Φc = 
was shown to be related to the root mean square displacement at long diffusion times by the simple relationship Φ
= 10λ2. In the range of diffusion times 100–530 msec, λ was found to be independent of the diffusion time and equal to 1.35 ± 0.22 μm and Φc to 4.27 ± 0.71 μm. The data reinforce the notion that the presence of lipid resonances in NMR spectra of tumors is due to lipid droplets. Light microscopy of histologic slices showed the presence of lipid droplets mainly in the necrotic region and in a layer of tumor cells surrounding the necrosis. Magn Reson Med 45:409–414, 2001. © 2001 Wiley-Liss, Inc.
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