High-resolution deletion mapping of chromosome arm 1p in pancreatic cancer identifies a major consensus region at 1p35
Werner Hilgers
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorDavid J. Tang
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorAvrahom Y. Sugar
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorManu C. Shekher
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorRalph H. Hruban
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorCorresponding Author
Scott E. Kern
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
632 Ross Bldg., The Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205.Search for more papers by this authorWerner Hilgers
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorDavid J. Tang
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorAvrahom Y. Sugar
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorManu C. Shekher
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorRalph H. Hruban
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorCorresponding Author
Scott E. Kern
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, Maryland
632 Ross Bldg., The Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205.Search for more papers by this authorAbstract
Chromosomal arm 1p has long been suspected, on the basis of loss of heterozygosity (LOH) and other data, to harbor a tumor suppressor gene important in pancreatic carcinomas and other tumors. We constructed a high-resolution map of LOH at 1p in a panel of pancreatic adenocarcinomas. Using 44 markers, we identified LOH on 1p in 49% of 43 cancers. Breakpoints in 1p were identified in 15 of the carcinomas and could be used to ascertain consensus patterns. We found a major consensus region of LOH at 1p35 between loci D1S233 and D1S247. This region participates in the majority of LOH events on 1p in pancreatic cancer. These data provide a roadmap for further regional mapping, homozygous deletion searches, comparison to LOH patterns seen in other tumor types, and prioritization of studies using candidate genes. Genes Chromosomes Cancer 24:351–355, 1999. © 1999 Wiley-Liss, Inc.
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