Tetrapeptide DEVD-aldehyde or YVAD-chloromethylketone inhibits Fas/Apo-1 (CD95)-mediated apoptosis in renal-cell-cancer cells
Corresponding Author
Yoshihiko Tomita
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Department of Urology. Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan. Fax: +81-25-229-2258Search for more papers by this authorTakashi Kawasaki
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Search for more papers by this authorVladimir Bilim
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Search for more papers by this authorMasayuki Takeda
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Search for more papers by this authorKota Takahashi
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Search for more papers by this authorCorresponding Author
Yoshihiko Tomita
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Department of Urology. Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan. Fax: +81-25-229-2258Search for more papers by this authorTakashi Kawasaki
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Search for more papers by this authorVladimir Bilim
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Search for more papers by this authorMasayuki Takeda
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Search for more papers by this authorKota Takahashi
Department of Urology, Niigata University School of Medicine, Asahimachi 1, Niigata 951, Japan
Search for more papers by this authorAbstract
The apoptotic machinery has been intensively investigated, and interleukin-l-beta-converting enzyme (ICE) and its homologs directly mediate apoptosis by means of their unique protease activity. Fas/Apol (CD95), a member of the TNF-receptor family, mediates apoptosis by binding to its ligand, which is mainly expressed on lymphocytes. Here, we investigated the expression and function of both molecules in renal-cell cancer (RCC). The expression of Fas was examined in 6 RCC cell lines by immunoblotting and all of them expressed Fas. ICE and CPP32/YAMA were also identified among the cell lines. We earlier examined ACHN cells expressing low levels of BCL-2, as well as KRC/Y cells with high levels of BCL-2. Here, we found that the anti-Fas monoclonal antibody, CH-11, induced apoptosis in a dose-dependent fashion more remarkably in ACHN cells. Pre-incubation with the tetrapeptide YVAD-chloromethylketone or DEVD-aldehyde inhibited Fas-mediated apoptosis. These findings suggest that, in RCC, apoptosis is induced by lymphocytes bearing Fas-L, and that it is achieved through the proteolytic action of CPP32/YAMA and/or ICE, or another member of the ICE/ced-3 protease family. © 1996 Wiley-Liss, Inc.
References
- Casciola-Rosen, L. A., Miller, D. K., Anhalt, G. J. and Rosen, A., Specific cleavage of the 70-kDa protein component of the U1 small nuclear ribonucleoprotein is a characteristic biochemical feature of apoptotic cell death. J. biol. Chem., 269, 30757–30760 (1994).
- Chinnaiyan, A. M., O'Rourke, K., Tewari, M. and Dixit, V. M., FADD, a novel death-domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. Cell, 81, 505–512 (1995).
- Chinnaiyan, A. M., Orth, K., O'Rourke, K., Duan, H., Poirier, G. G. and Dixit, V. M., Molecular ordering of the cell-death pathway. J. biol. Chem., 271, 4573–4576 (1996).
- Ellis, R. E., Yuan, J. Y. and Horvitz, H. R., Mechanisms and functions of cell death. Ann. Rev. Cell Biol., 7, 663–698 (1991).
- Enari, M., Hug, H. and Nagata, S., Involvement of an ICE-like protease in Fas-mediated apoptosis. Nature (Lond.), 375, 78–81 (1995).
- Faucheu, C. and 14 others, A novel human protease similar to the interleukin-1-beta-converting enzyme induces apoptosis in transfected cells. EMBO J., 14, 1914–1922 (1995).
- Fernandes-Alnemri, T., Litwack, G. and Alnemri, E. S., CPP32, a novel human aoptotic protein with homology to Caenorhabditis elegans cell-death protein Ced-3 and mammalian interleukin-1-betaconverting enzyme. J. biol. Chem., 269, 30761–30764 (1994).
- Fernandes-Alnemri, T., Litwack, G. and Alnemri, E. S., Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family. Cancer Res., 55, 2737–2742 (1995).
- Graham, S. D. J., Immunotherapy of renal-cell carcinoma. Probl. Urol., 8, 575–596 (1994).
- Ju, S. T., Panka, D. J., Cui, H., Ettinger, R., El-Khatib, M., Sherr, D. H., Stanger, B. Z. and Marshak-Rothstein, A., Fas(CD95)/FasL interactions required for programmed cell death after T-cell activation. Nature (Lond.), 373, 444–448 (1995).
- Kamens, J. and 12 others, Identification and characterization of ICH-2, a novel member of the interleukin-1-beta-converting enzyme family of cysteine proteases. J. biol. Chem., 270, 15250–15256 (1995).
- Kaufmann, S. H., Desnoyers, S., Ottaviano, Y., Davidson, N. E. and Poirier, G. G., Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res., 53, 3976–3985 (1993).
- Kuida, K., Lippke, J. A., Ku, G., Harding, M. W., Livingston, D. J., Su, M. S. and Flavell, R. A., Altered cytokine export and apoptosis in mice deficient in interleukin-1-beta-converting enzyme. Science, 267, 2000–2003 (1995).
- Kumar, S., Kinoshita, M., Noda, M., Copeland, N. G. and Jenkins, N. A., Induction of apoptosis by the mouse Nedd2 gene, which encodes a protein similar to the product of the Caenorhabditis elegans cell-death gene ced-3 and the mammalian IL-1-beta-converting enzyme. Genes Dev., 8, 1613–1626 (1994).
- Lazebnik, Y. A., Kaufmann, S. H., Desnoyers, S., Poirier, G. G. and Earnshaw, W. C., Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature (Lonnd), 371, 346–347 (1994).
- Li, P. and 16 others, Mice deficient in IL-1-beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock. Cell, 80, 401–411 (1995).
- Maartin, S. J., O'Brien, G. A., Nishioka, W. K., McGahon, A. J., Mahoubi, A., Saido, T. C. and Green, D. R., Proteolysis of fodrin (non-erythroid spectrin) during apoptosis. J. biol. Chem., 270, 6425–6428 (1995).
- Miura, M., Zhu, H., Rotello, R., Hartwieg, E. A. and Yuan, J., Induction of apoptosis in fibroblasts by IL-1-beta-converting enzyme, a mammalian homolog of the C. elegans cell-death gene ced-3. Cell, 75, 653–660 (1993).
- Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. immunol. Methods, 65, 55–63 (1983).
- Munday, N. A., Vaillancourt, J. P., Ali, A., Casano, F. J., Miller, D. K., Molineaux, S. M., Yamin, T. T., Yu, V. L. and Nicholson, D. W., Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases. J. biol. Chem., 270, 15870–15876 (1995).
- Nagata, S., Fas and Fas ligand: a death factor and its receptor. Advanc. Immunol., 57, 129–144 (1994).
- Nagata, S. and Golstein, P., The Fas death factor. Science, 267, 1449–1456 (1995).
- Neamati, N., Fernandez, A., Wright, S., Kiefer, J. and McConkey, D. J., Degradation of lamin B1 precedes oligonucleosomal DNA fragmentation in apoptotic thymocytes and isolated thymocyte nuclei. J. Immunol., 154, 3788–3795 (1995).
- Nicholson, D. W. and 15 others, Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature (Lond.), 376, 37–43 (1995).
- Schlegel, J., Peters, I., Orrenius, S., Miller, D. K., Thornberry, N. A., Yamin, T. T. and Nicholson, D. W., CPP32/apopain is a key interleukin-1-beta-converting enzyme-like protease involved in Fasmediated apoptosis. J. biol. Chem., 271, 1841–1844 (1996).
- Seamus, J. and Green, D. R., Protease activation during apoptosis; death by thousand cuts? Cell, 82, 349–352 (1995).
- Suzuki, Y., Tamura, G., Satodate, R. and Fujioka, T., Infrequent mutation of p53 gene in human renal-cell carcinoma detected by polymerase-chain-reaction single-strand conformation polymorphism analysis. Jap. J. Cancer Res., 83, 233–235 (1992).
- Takahashi, T., Tanaka, M., Inazawa, J., Abe, T., Suda, T. and Nagata, S., Human Fas ligand: gene structure, chromosomal location and species specificity. Int. Immunol., 6, 1567–1574 (1994).
- Tewari, M., Quan, L. T., O'Rourke, K., Desnoyers, S., Zeng, Z., Beidler, D. R., Poirier, G. G., Salvesen, G. S. and Dixit, V. M., Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell, 81, 801–809 (1995).
- Thornberry, N. A. and 28 others, A novel heterodimeric cysteine protease is required for interleukin-1-beta processing in monocytes. Nature (Lond.), 356, 768–774 (1992).
-
Tomita, Y.,
Bilim, V.,
Kawasaki, T.,
Takeda, M.,
Kota, T.,
Ismail, O.,
Magnusson, K. and
Wiman, K.,
Frequent expression of bcl-2 in renal-cell carcinomas carrying wild-type p53
Int. J. Cancer,
66, 322–325
(1996).
10.1002/(SICI)1097-0215(19960503)66:3<322::AID-IJC9>3.0.CO;2-0 PubMed Web of Science® Google Scholar
- Voelkel-Johnson, C., Entingh, A. J., Wold, W. S., Gooding, L. R. and Laster, S. M., Activation of intracellular roteases is an early event in TNF-induced apoptosis. J. Immunol., 154, 1707–1716 (1995).
- Wang, L., Miura, M., Bergeron, L., Zhu, H. and Yuan, J., Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death. Cell, 78, 739–750 (1994).
- Williams, M. S. and Henkart, P. A., Apoptotic cell death induced by intracellular proteolysis. J. Immunol., 153, 4247–4255 (1994).
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