Volume 2019, Issue 1 4254759

Research Article

Open Access

Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells

Tian Hu

School of Medicine, Nankai University, Tianjin 300052, China nankai.edu.cn

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China 301hospital.com.cn

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Yongde Xu,

Yongde Xu

Department of Urology, Beijing Friendship Hospital, The Capital University of Medical Sciences, Beijing 100050, China bfh.com.cn

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Bin Yao,

Bin Yao

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China 301hospital.com.cn

Key Laboratory of Tissue Repair and Regeneration of PLA, Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, First Hospital Affiliated to General Hospital of PLA, Beijing 100048, China 301hospital.com.cn

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Xiaobing Fu,

Corresponding Author

Xiaobing Fu

[email protected]

orcid.org/0000-0002-2465-1700

School of Medicine, Nankai University, Tianjin 300052, China nankai.edu.cn

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China 301hospital.com.cn

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Sha Huang,

Corresponding Author

Sha Huang

[email protected]

orcid.org/0000-0001-8868-2680

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China 301hospital.com.cn

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Tian Hu,

Tian Hu

School of Medicine, Nankai University, Tianjin 300052, China nankai.edu.cn

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China 301hospital.com.cn

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Yongde Xu,

Yongde Xu

Department of Urology, Beijing Friendship Hospital, The Capital University of Medical Sciences, Beijing 100050, China bfh.com.cn

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Bin Yao,

Bin Yao

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China 301hospital.com.cn

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Xiaobing Fu,

Corresponding Author

Xiaobing Fu

[email protected]

orcid.org/0000-0002-2465-1700

School of Medicine, Nankai University, Tianjin 300052, China nankai.edu.cn

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China 301hospital.com.cn

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Sha Huang,

Corresponding Author

Sha Huang

[email protected]

orcid.org/0000-0001-8868-2680

Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, China 301hospital.com.cn

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First published: 04 February 2019

https://doi.org/10.1155/2019/4254759

Citations: 5

Academic Editor: Cinzia Marchese

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Abstract

Sweat glands developed from the embryonic epidermis. To elucidate the underlying mechanisms of morphogenesis, a reliable in vitro test system for bioactive screening must be developed. Here, we described a novel and convenient model by coculturing embryonic tissue and epidermal stem cells (ESCs) using Transwell insert for evaluating the effects of soluble morphogens on sweat gland morphogenesis in vitro. Using this coculture system, morphological alteration, histological features, and specific markers were observed. Initial experiments revealed that ESCs cocultured with embryonic paw pad (EPP) tissue demonstrated glandular structure and cytokeratin 8 (K8) and cytokeratin 18 (K18) positive, while ESCs cocultured with embryonic dorsal skin demonstrated “sea snail” structure and K8, K18 negative. Moreover, bone morphogenetic protein 4 (BMP4) and epidermal growth factor (EGF) concentrations were detected in the medium of the EPP group. BMP receptor inhibitor could effectively block the ESC differentiation to sweat glands, while EGF receptor blocker did not show the effect. Our results showed clear benefits of this novel and convenient model in terms of in vitro-in vivo correlation. It was an appropriate alternative for screening of potential bioactives regulating the sweat gland morphogenesis mechanism.

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Data Availability

All data are fully available without restriction. All data were included in the paper.

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