Predisposing factors for anti-D immune response in D⁻ patients with chronic liver disease transfused with D⁺ platelet concentrates

BACKGROUND

Recent reports have indicated that the risk of anti-D alloimmunization following D-incompatible platelet (PLT) transfusion is low in hematology and oncology patients. We investigated the rate of anti-D alloimmunization in RhD-negative (D⁻) patients with chronic liver disease transfused with D⁺ platelet concentrates (PCs) and the factors involved, at a liver transplant (LT) center.

STUDY DESIGN AND METHODS

We reviewed the blood bank database from January 2003 to October 2016.

D⁻ patients who had received D⁺ PLT transfusions were eligible if they had undergone antibody screening at least 28 days after the first D⁺ PC transfusion, had no previous or concomitant exposure to D⁺ blood products, and had not received anti-D immunoglobulins.

RESULTS

Six of the 56 eligible patients (10.7%) had anti-D antibodies. All had received whole blood-derived PCs. Four of 20 patients (20%) untransplanted or transfused before LT and only two of 36 patients (5.6%) transfused during or after LT produced anti-D antibodies. These two patients were on maintenance immunosuppression based on low-dose steroids and tacrolimus. The factors identified as significantly associated with anti-D immune response were the presence of red blood cell immune alloantibodies before D⁺ PLT transfusion (p = 0.003), and D⁺ PLT transfusion outside the operative and postoperative (5 days) periods for LT (p = 0.023).

CONCLUSION

D⁻ patients with chronic liver disease transfused with D⁺ PLTs before LT are at high risk of developing anti-D antibodies. Preventive measures should be considered for these patients.