Phenotypic and genotypic characterization of salmonella Enteritidis isolated from two consecutive Food-Poisoning outbreaks in Sichuan, China
Ximei Kong
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorJingxian Chen
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Search for more papers by this authorYang Yang
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorMing Li
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorJian Wang
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Search for more papers by this authorQu Jia
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Search for more papers by this authorYao Wang
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorQiwu Yuan
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorYanfang Miao
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorPinnan Zhao
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorYiping You
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorXing Zhao
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Search for more papers by this authorXiaofang Pei
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Search for more papers by this authorCorresponding Author
Haojiang Zuo
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Correspondence
Haojiang Zuo, Department of Public Health Laboratory Sciences, West China School of Public Health and West China Fourth Hospital, Sichuan University. Renmin South Road 3rd Section NO.16, Chengdu 610041, Sichuan, China.
Email: [email protected]; [email protected]
Jiantong Meng, Department of Microbiology Laboratory, Chengdu Center for Disease Control and Prevention, Long Xiang Road NO.4, 610041, Chengdu, Sichuan, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jiantong Meng
Chengdu Center for Disease Control and Prevention, Chengdu, China
Correspondence
Haojiang Zuo, Department of Public Health Laboratory Sciences, West China School of Public Health and West China Fourth Hospital, Sichuan University. Renmin South Road 3rd Section NO.16, Chengdu 610041, Sichuan, China.
Email: [email protected]; [email protected]
Jiantong Meng, Department of Microbiology Laboratory, Chengdu Center for Disease Control and Prevention, Long Xiang Road NO.4, 610041, Chengdu, Sichuan, China.
Email: [email protected]
Search for more papers by this authorXimei Kong
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorJingxian Chen
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Search for more papers by this authorYang Yang
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorMing Li
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorJian Wang
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Search for more papers by this authorQu Jia
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Search for more papers by this authorYao Wang
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorQiwu Yuan
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorYanfang Miao
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorPinnan Zhao
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorYiping You
Chengdu Center for Disease Control and Prevention, Chengdu, China
Search for more papers by this authorXing Zhao
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Search for more papers by this authorXiaofang Pei
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Search for more papers by this authorCorresponding Author
Haojiang Zuo
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China
Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, Chengdu, China
Correspondence
Haojiang Zuo, Department of Public Health Laboratory Sciences, West China School of Public Health and West China Fourth Hospital, Sichuan University. Renmin South Road 3rd Section NO.16, Chengdu 610041, Sichuan, China.
Email: [email protected]; [email protected]
Jiantong Meng, Department of Microbiology Laboratory, Chengdu Center for Disease Control and Prevention, Long Xiang Road NO.4, 610041, Chengdu, Sichuan, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jiantong Meng
Chengdu Center for Disease Control and Prevention, Chengdu, China
Correspondence
Haojiang Zuo, Department of Public Health Laboratory Sciences, West China School of Public Health and West China Fourth Hospital, Sichuan University. Renmin South Road 3rd Section NO.16, Chengdu 610041, Sichuan, China.
Email: [email protected]; [email protected]
Jiantong Meng, Department of Microbiology Laboratory, Chengdu Center for Disease Control and Prevention, Long Xiang Road NO.4, 610041, Chengdu, Sichuan, China.
Email: [email protected]
Search for more papers by this authorXimei Kong Jingxian Chen contributed equally to this work.
Funding information: Sichuan University; Department of Science and Technology of Sichuan Province, Grant/Award Number: 2019YJ0018; Chengdu Science and Technology Bureau, Grant/Award Number: 2019-YF05-01247-SN; Yibin Science and Technology Bureau, Grant/Award Number: 2021NY006
Abstract
Salmonella enterica serotype Enteritidis (SE) is a primary pathogen that causes foodborne diseases in humans. Although whole-genome sequencing (WGS) -based typing analyses have been increasingly used to investigate food-poisoning outbreaks, they are rarely applied to the epidemiology of multiple Salmonella outbreaks in Sichuan, China. This study therefore isolated SE from patients and food of two consecutive food-poisoning outbreaks during 2020 in Sichuan, China. We tracked outbreak origin using epidemiological investigation, serotyping, antimicrobial susceptibility testing (AST), pulsed-field gel electrophoresis (PFGE), and WGS. We also determined phylogenetic relationships using PFGE, whole and core genome multilocus sequence typing (wg/cgMLST), and whole-genome single nucleotide polymorphism (wgSNP) analyses. Epidemiological investigations identified a correlation between cake consumption and food poisoning. Thirteen strains isolated from patients and one strain isolated from the cake were confirmed as SE. Among the 14 strains, only six shared the same AST pattern (AMP-AMS-Sul-STR). Isolates from patients and cakes were indistinguishable in PFGE results. All four methods, namely PFGE, wgMLST, cgMLST, and wgSNP were appropriate for bacterial typing in SE-related outbreak investigation. However, wgSNP can assign 12 SE strains from the first outbreak to one cluster and assign two SE strains from the second outbreak to another cluster, while PFGE, wgMLST, cgMLST did not successfully distinguish the SE strains from different outbreaks. Thus, we conclude that SNP-based phylogenetic analysis might be a viable method for differentiating SE strains at the outbreak level.
CONFLICT OF INTEREST
The authors have no conflict of interest to declare.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available on request from the corresponding author.
Supporting Information
| Filename | Description |
|---|---|
| jfs13015-sup-0001-Supinfo01.docxWord 2007 document , 80.2 KB | File S1 WgMLST loci of 14 Salmonella isolates. |
| jfs13015-sup-0002-Supinfo02.docxWord 2007 document , 79.1 KB | File S2 cgMLST loci of 14 Salmonella isolates. |
| jfs13015-sup-0003-Supinfo03.docxWord 2007 document , 17.4 KB | File S3 the SNP divergence among 14 Salmonella isolates. |
| jfs13015-sup-0004-Supinfo04.pdfPDF document, 108.9 KB | File S4 FilmArray GI PCR panel detection result_1 (PDF). |
| jfs13015-sup-0005-Supinfo05.pdfPDF document, 109 KB | File S5 FilmArray GI PCR panel detection result_2 (PDF). |
| jfs13015-sup-0006-Supinfo06.pdfPDF document, 110 KB | File S6 FilmArray GI PCR panel detection result_3 (PDF). |
| jfs13015-sup-0007-Supinfo07.pdfPDF document, 109.6 KB | File S7 FilmArray GI PCR panel detection result_4 (PDF). |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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