Comprehensive proteome and lysine acetylome analysis after artificial aging reveals the key acetylated proteins involved in wheat seed oxidative stress response and energy production
Bang-Bang Li
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorWei Zhang
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorShan Wei
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorYang-Yong Lv
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorJi-Xu Shang
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorCorresponding Author
Yuan-Sen Hu
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Correspondence
Yuan-Sen Hu, College of Biological Engineering, Henan University of Technology, Zhengzhou, China.
Email: [email protected]
Search for more papers by this authorBang-Bang Li
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorWei Zhang
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorShan Wei
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorYang-Yong Lv
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorJi-Xu Shang
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Search for more papers by this authorCorresponding Author
Yuan-Sen Hu
College of Biological Engineering, Henan University of Technology, Zhengzhou, China
Correspondence
Yuan-Sen Hu, College of Biological Engineering, Henan University of Technology, Zhengzhou, China.
Email: [email protected]
Search for more papers by this authorAbstract
Lysine acetylation is a common post-translational modification of proteins within all organisms. However, quantitative acetylome characterization in wheat seed during aging in storage has not been reported. This study reports the first large-scale acetylome analysis of wheat seeds after artificial aging treatment, using the quantitative proteomic approach. In total, 11,002 acetylation sites, corresponding to 4262 acetylated proteins were identified, of which 1207 acetylated sites, representing 783 acetylated proteins, were significantly more or less acetylated after artificial aging. Functional analysis demonstrated that the majority of the acetylated proteins are closely involved with cellular and metabolic functions. In particular, key enzymes in the oxidative stress response and energy metabolism were significantly differentially acetylated and appear to be heavily involved in wheat seed aging. The acetylome analysis was verified by quantitative real-time PCR and enzyme activity determination. Lysine-acetylation results in a weaker oxidative stress response and lower energy production efficiency, resulting in the apoptosis of wheat seed cells, insufficient energy supply at the germination stage, and consequently, marked loss of seed vigor.
Practical applications
It is known that the loss of protein function is an important reason for the decrease of seed vigor. Therefore, the change of protein function in the process of wheat seed aging was studied by proteome and lysine acetylome analysis technology. The results showed that the oxidation–reduction imbalance and the decrease of energy production efficiency of seeds were the important reasons for the decrease of their vigor. This will provide a new idea for green and safe storage of grain.
CONFLICT OF INTEREST
The authors have declared no conflict of interest for this article.
Supporting Information
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