The synergistic effect of Angelica sinensis (Oliv.) Diels and Rehmannia glutinosa (Gaertn.) DC. on antioxidant activity and protective ability against cell injury
Qingying Zhang
Chemistry and Materials School, Jinan University, Guangzhou, China
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China
Contribution: Conceptualization, Data curation, Formal analysis, Methodology, Software, Validation, Visualization, Writing - original draft, Writing - review & editing
Search for more papers by this authorTianzhi Yang
Chemistry and Materials School, Jinan University, Guangzhou, China
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China
Contribution: Investigation, Resources, Software
Search for more papers by this authorDongmei Li
Chemistry and Materials School, Jinan University, Guangzhou, China
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China
Contribution: Software, Validation
Search for more papers by this authorMengyu Ma
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Investigation
Search for more papers by this authorXiaoling Liang
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Resources
Search for more papers by this authorZixing Ma
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Investigation
Search for more papers by this authorQianglong Ye
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Investigation
Search for more papers by this authorHantao Yang
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Visualization
Search for more papers by this authorMinghui Li
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Visualization
Search for more papers by this authorCorresponding Author
Ailan Qu
Chemistry and Materials School, Jinan University, Guangzhou, China
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China
Correspondence
Ailan Qu and Yao Chen, Chemistry and Materials School, Jinan University, Guangzhou 510632, China.
Email: [email protected] (A. Q.) and Email: [email protected] (Y. C.)
Contribution: Funding acquisition, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorCorresponding Author
Yao Chen
Chemistry and Materials School, Jinan University, Guangzhou, China
Correspondence
Ailan Qu and Yao Chen, Chemistry and Materials School, Jinan University, Guangzhou 510632, China.
Email: [email protected] (A. Q.) and Email: [email protected] (Y. C.)
Contribution: Supervision, Writing - review & editing
Search for more papers by this authorQingying Zhang
Chemistry and Materials School, Jinan University, Guangzhou, China
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China
Contribution: Conceptualization, Data curation, Formal analysis, Methodology, Software, Validation, Visualization, Writing - original draft, Writing - review & editing
Search for more papers by this authorTianzhi Yang
Chemistry and Materials School, Jinan University, Guangzhou, China
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China
Contribution: Investigation, Resources, Software
Search for more papers by this authorDongmei Li
Chemistry and Materials School, Jinan University, Guangzhou, China
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China
Contribution: Software, Validation
Search for more papers by this authorMengyu Ma
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Investigation
Search for more papers by this authorXiaoling Liang
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Resources
Search for more papers by this authorZixing Ma
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Investigation
Search for more papers by this authorQianglong Ye
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Investigation
Search for more papers by this authorHantao Yang
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Visualization
Search for more papers by this authorMinghui Li
Chemistry and Materials School, Jinan University, Guangzhou, China
Contribution: Visualization
Search for more papers by this authorCorresponding Author
Ailan Qu
Chemistry and Materials School, Jinan University, Guangzhou, China
Singwong Asia Pacific and Jinan University Joint R & D Center, Guangzhou, China
Correspondence
Ailan Qu and Yao Chen, Chemistry and Materials School, Jinan University, Guangzhou 510632, China.
Email: [email protected] (A. Q.) and Email: [email protected] (Y. C.)
Contribution: Funding acquisition, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorCorresponding Author
Yao Chen
Chemistry and Materials School, Jinan University, Guangzhou, China
Correspondence
Ailan Qu and Yao Chen, Chemistry and Materials School, Jinan University, Guangzhou 510632, China.
Email: [email protected] (A. Q.) and Email: [email protected] (Y. C.)
Contribution: Supervision, Writing - review & editing
Search for more papers by this authorAbstract
Oxidative stress-induced dysfunction of nerve cells has been implicated as a crucial cause of cell death in neurodegenerative diseases. In Asian countries, herbs, such as Angelica sinensis (Oliv.) Diels (DG) and Rehmannia glutinosa (Gaertn.) DC. (SDH), have long be considered to have antiaging abilities. The herbs act as neuro protectants that rescue nerve cells from oxidative stress damage and apoptosis. Thus, developing herbal formulas can potentially lead to new treatments for neurodegenerative diseases. In this study, we compared the effective active components and antioxidant properties of extractive of DG and SDH (DG-SDH) when formulated at different ratios. DG-SDH formulated at a ratio of 3:2 (DG-SDH [3:2]) produced the highest content of polysaccharides, polyphenols, and flavonoids. It also showed the best ability in removing DPPH and hydroxyl free radicals compared to single herb or other compounding ratio. The antioxidant activity of DG-SDH (3:2) showed best synergistic effects in scavenging activity assays of DPPH free radicals and hydroxyl free radicals. DG-SDH (3:2) could increase the cell viability of SHSY-5Y cells, PC-12 cells, and BV-2 cells. In particular, DG-SDH (3:2) protected SHSY-5Y cells from H2O2-induced cell injury by inhibiting excessive expression of reactive oxygen species (ROS), reducing the rate of apoptosis and restoring mitochondrial membrane potential. Actin-Tracker Green and DAPI staining and fluorescence microscope observation confirmed that DG-SDH (3:2) helped in preserving cell morphology under oxidative stress. These findings support that DG-SDH (3:2) promote the neuroprotection against hydrogen peroxide and can serve as a novel therapy for neurodegenerative diseases.
Practical applications
This is the first study to investigate DG and SDH interaction between effective ingredients. These findings support that DG-SDH (3:2) has the best synergistic effects in antioxidant activity and promote the neuroprotection against hydrogen peroxide. Hence, DG-SDH (3:2) will be an excellent candidate to be developed as a functional food ingredients or nutraceuticals for neurodegenerative diseases.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| jfbc14196-sup-0001-supinfo.docxWord 2007 document , 317 KB |
Appendix S1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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