The immune-enhancing effect and in vitro antioxidant ability of different fractions separated from Colla corii asini
Guowei Zhang
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Data curation, Formal analysis, Methodology, Writing - original draft
Search for more papers by this authorFengxian Guo
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China
Contribution: Investigation, Validation, Visualization
Search for more papers by this authorMaomao Zeng
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Resources, Software, Validation
Search for more papers by this authorZhaojun Wang
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Software, Validation, Visualization
Search for more papers by this authorFang Qin
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Formal analysis, Investigation, Methodology
Search for more papers by this authorJie Chen
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Conceptualization, Formal analysis, Investigation
Search for more papers by this authorCorresponding Author
Zongping Zheng
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China
Correspondence
Zongping Zheng and Zhiyong He, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, Fujian, China.
Email: [email protected] (Z. Z.) and Email: [email protected] (Z. H.)
Contribution: Resources, Validation, Visualization
Search for more papers by this authorCorresponding Author
Zhiyong He
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Correspondence
Zongping Zheng and Zhiyong He, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, Fujian, China.
Email: [email protected] (Z. Z.) and Email: [email protected] (Z. H.)
Contribution: Investigation, Methodology, Supervision, Writing - review & editing
Search for more papers by this authorGuowei Zhang
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Data curation, Formal analysis, Methodology, Writing - original draft
Search for more papers by this authorFengxian Guo
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China
Contribution: Investigation, Validation, Visualization
Search for more papers by this authorMaomao Zeng
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Resources, Software, Validation
Search for more papers by this authorZhaojun Wang
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Software, Validation, Visualization
Search for more papers by this authorFang Qin
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Formal analysis, Investigation, Methodology
Search for more papers by this authorJie Chen
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Conceptualization, Formal analysis, Investigation
Search for more papers by this authorCorresponding Author
Zongping Zheng
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China
Correspondence
Zongping Zheng and Zhiyong He, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, Fujian, China.
Email: [email protected] (Z. Z.) and Email: [email protected] (Z. H.)
Contribution: Resources, Validation, Visualization
Search for more papers by this authorCorresponding Author
Zhiyong He
Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Correspondence
Zongping Zheng and Zhiyong He, Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou 362000, Fujian, China.
Email: [email protected] (Z. Z.) and Email: [email protected] (Z. H.)
Contribution: Investigation, Methodology, Supervision, Writing - review & editing
Search for more papers by this authorGuowei Zhang and Fengxian Guo contributed equally to this study.
Abstract
In this study, Colla corii asini (CCA) was fractionated into three fractions with different molecular weights using ultracentrifugation equipment. Components with a molecular weight of >10 kDa in F1 accounted for 81.90%, whereas that in F2 and F3 was 15.63% and 0.94%, respectively. The immunomodulatory activity of CCA fractions was investigated using RAW264.7 cell model and their antioxidant abilities were evaluated by 2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) and ferric-reducing antioxidant power (FRAP) assay. The results indicated that RAW264.7 cells treated with F1 released the highest level of nitric oxide, reactive oxygen species, interleukin-6, and tumor necrosis factor-α. The ABTS and FRAP value of F1 were 65.81% and 29.33 μM TE/L, respectively, which were 22.53%, 128.44% and 43.72%, 132.16% higher than that of F2 and F3, respectively. These results suggested that components with a molecular weight of >10 kDa in CCA had stronger immunomodulatory and antioxidant ability, which would help develop the health food based on CCA.
Practical applications
Colla corii asini (CCA) is a famous protein-based traditional Chinese medicine and nutritional supplement. During the processing of CCA, the molecular weight (MW) of CCA collagen components changed dynamically due to the protein aggregation, degradation, and the Maillard reaction. Some studies have shown that the MW distribution of CCA was not uniform. However, the MW range of CCA components which has strong antioxidant and immunomodulatory activity is still not clear, and few studies have reported the mechanism of CCA's immunomodulatory activity and active ingredients. Therefore, it is important to figure out the characteristics of CCA components with stronger immunomodulatory and antioxidant ability, such as the MW distribution and chemical composition of CCA fractions. And this study will be great for the processing of CCA products which has better biological functions.
CONFLICT OF INTEREST
The authors declare that they have no conflicts of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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