The synergistic effect of cell wall extracted from probiotic biomass containing Lactobacillus acidophilus CL1285, L. casei LBC80R, and L. rhamnosus CLR2 on the anticancer activity of cranberry juice—HPLC fractions
Kerlynn Desrouillères
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Search for more papers by this authorMathieu Millette
Bio-K Plus International Inc., Laval, QC, Canada
Search for more papers by this authorLeila Bagheri
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Search for more papers by this authorBehnoush Maherani
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Search for more papers by this authorMajid Jamshidian
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Search for more papers by this authorCorresponding Author
Monique Lacroix
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Correspondence
Monique Lacroix, Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, 531 Blvd. des Prairies, Laval, Quebec, H7V 1B7, Canada
Email: [email protected]
Search for more papers by this authorKerlynn Desrouillères
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Search for more papers by this authorMathieu Millette
Bio-K Plus International Inc., Laval, QC, Canada
Search for more papers by this authorLeila Bagheri
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Search for more papers by this authorBehnoush Maherani
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Search for more papers by this authorMajid Jamshidian
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Search for more papers by this authorCorresponding Author
Monique Lacroix
Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, Laval, QC, Canada
Correspondence
Monique Lacroix, Research Laboratories in Sciences Applied to Food, Canadian Irradiation Center, INRS, Armand-Frappier, Health and Biotechnology Center, Institute of Nutraceutical and Functional Foods, 531 Blvd. des Prairies, Laval, Quebec, H7V 1B7, Canada
Email: [email protected]
Search for more papers by this authorAbstract
Anticancer effects were evaluated on three HPLC fractions obtained from a concentrated cranberry juice and cell wall constituents extracted from a probiotic biomass containing Lactobacillus acidophilus CL1285, Lactobacillus casei LBC80R, and Lactobacillus rhamnosus CLR2. The samples were tested at increasing concentrations for the antiproliferative assay using HT-29 cells' line and for the quinone reductase (QR) assay using Hepa-1c1c7 murine hepatoma cells. Fraction 1 (F1) which is highly concentrated with phenolic acids inhibited the growth of the HT-29 cells' line with IC50 values of 14.80 µg Gallic acid equivalent (GAE)/ml. The fraction 3 (F3) which is highly concentrated in flavonols had potency as QR inducer. Furthermore, the results showed that all cranberry fractions combined with cell wall constituents extracted from the probiotic biomass were more effective in inhibiting the growth of HT-29 as compared to the cranberry fractions tested alone, indicating a possible synergy effect between these bio-functional compounds.
Practical applications
This study strongly evidenced that cranberry juice fractions combined with cell wall constituents extracted from the probiotic biomass can be used as a potent preventive functional compound against colorectal cancer. Therefore, this research proposes a natural dietary compound to prevent mutagenesis and carcinogenesis of colorectal cancer. Furthermore, the industry can formulae products containing probiotic and phenolic compounds as colon cancer cell growth preventive and anticancer products.
CONFLICT OF INTEREST
The authors declare no conflict of interest in this study.
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