Effect of polysaccharide extract SPSS1 from Apostichopus japonicus spermary on HepG2 cells via iTRAQ-based proteome analysis
Correction(s) for this article
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Effect of polysaccharide extract SPSS1 from Apostichopus japonicus spermary on HepG2 cells via iTRAQ-based proteome analysis
- Volume 44Issue 7Journal of Food Biochemistry
- First Published online: July 6, 2020
Corresponding Author
Jian Zhang
Shandong Marine Resource and Environment Research Institute, Yantai, China
College of Biotechnology, The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
Correspondence
Jian Zhang, Shandong Marine Resource and Environment Research Institute, No. 216 Changjiang Rd., Yantai Economic & Technical Development Zone, Yantai, Shandong 264006, China.
Email: [email protected]
Search for more papers by this authorShaowei Liu
College of Biotechnology, The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
Search for more papers by this authorLaihao Li
Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, Guangzhou, China
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China
Search for more papers by this authorGongming Wang
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorFang Liu
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorYunping Zhao
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorYuexin Jing
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorZhenduo Li
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorCorresponding Author
Jian Zhang
Shandong Marine Resource and Environment Research Institute, Yantai, China
College of Biotechnology, The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
Correspondence
Jian Zhang, Shandong Marine Resource and Environment Research Institute, No. 216 Changjiang Rd., Yantai Economic & Technical Development Zone, Yantai, Shandong 264006, China.
Email: [email protected]
Search for more papers by this authorShaowei Liu
College of Biotechnology, The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
Search for more papers by this authorLaihao Li
Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, Guangzhou, China
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China
Search for more papers by this authorGongming Wang
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorFang Liu
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorYunping Zhao
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorYuexin Jing
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this authorZhenduo Li
Shandong Marine Resource and Environment Research Institute, Yantai, China
Search for more papers by this author[Correction added on 04 June 2020, after first online publication: in the article title, the word ‘japonicas’ was corrected to ‘japonicus’.]
Abstract
In this study, polysaccharide extract was prepared from Apostichopus japonicus spermary and purified by ion-exchange chromatography and gel filtration chromatography. Two main fractions named SPSS1 and SPSS2 were obtained and analyzed by ultraviolet spectroscopy and mixed with KBr, respectively. Chemical components analysis proved that SPSS1 and SPSS2 were rich in sulfate. Monosaccharide analysis indicated that in addition to the high content of lactose in both kinds of polysaccharides, the highest content of monosaccharide in SPSS1 was galactose, while in SPSS2 it was fucose. Further, the antitumor study of SPSS1 was carried and the results showed that SPSS1 treatment inhibited the proliferation of HepG2 cells. Through the iTRAQ-based proteome analysis, there were 208 differential proteins between control tumor cells and SPSS1 treatment of tumor cells. Compared to control tumor cells, 135 proteins were upregulated and 73 proteins were downregulated in treatment tumor cells.
Practical applications
Our study suggested that polysaccharide from sea cucumbers had the potential to be further developed as antitumor drugs.
CONFLICT OF INTEREST
There is no conflict of interest in the study.
Supporting Information
| Filename | Description |
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| jfbc13168-sup-0001-FigS1.tifTIFF image, 153.6 KB | Fig S1 |
| jfbc13168-sup-0002-FigS2.tifTIFF image, 260.8 KB | Fig S2 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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