Pyroptosis-related gene signature elicits immune response in rosacea
Corresponding Author
Xi-min Hu
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, China
Correspondence
Ji Li and Xi-min Hu, Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Email: [email protected] and [email protected]
Rong-hua Yang, Department of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou 510180, China.
Email: [email protected]
Search for more papers by this authorSheng-yuan Zheng
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorRui Mao
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorQi Zhang
Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, China
Search for more papers by this authorXin-xing Wan
Department of Endocrinology, Third Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorYi-ya Zhang
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Hunan Key Laboratory of Aging Biology, Xiangya Hospital, Central South University, Changsha, China
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorCorresponding Author
Ji Li
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Hunan Key Laboratory of Aging Biology, Xiangya Hospital, Central South University, Changsha, China
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Correspondence
Ji Li and Xi-min Hu, Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Email: [email protected] and [email protected]
Rong-hua Yang, Department of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou 510180, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Rong-hua Yang
Department of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China
Correspondence
Ji Li and Xi-min Hu, Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Email: [email protected] and [email protected]
Rong-hua Yang, Department of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou 510180, China.
Email: [email protected]
Search for more papers by this authorKun Xiong
Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, China
Hunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China
Key Laboratory of Emergency and Trauma, Ministry of Education, College of Emergency and Trauma, Hainan Medical University, Haikou, China
Search for more papers by this authorCorresponding Author
Xi-min Hu
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, China
Correspondence
Ji Li and Xi-min Hu, Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Email: [email protected] and [email protected]
Rong-hua Yang, Department of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou 510180, China.
Email: [email protected]
Search for more papers by this authorSheng-yuan Zheng
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorRui Mao
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorQi Zhang
Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, China
Search for more papers by this authorXin-xing Wan
Department of Endocrinology, Third Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorYi-ya Zhang
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Hunan Key Laboratory of Aging Biology, Xiangya Hospital, Central South University, Changsha, China
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Search for more papers by this authorCorresponding Author
Ji Li
Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China
Hunan Key Laboratory of Aging Biology, Xiangya Hospital, Central South University, Changsha, China
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China
Correspondence
Ji Li and Xi-min Hu, Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Email: [email protected] and [email protected]
Rong-hua Yang, Department of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou 510180, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Rong-hua Yang
Department of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China
Correspondence
Ji Li and Xi-min Hu, Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China.
Email: [email protected] and [email protected]
Rong-hua Yang, Department of Burn and Plastic Surgery, Guangzhou First People's Hospital, South China University of Technology, Guangzhou 510180, China.
Email: [email protected]
Search for more papers by this authorKun Xiong
Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, China
Hunan Key Laboratory of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China
Key Laboratory of Emergency and Trauma, Ministry of Education, College of Emergency and Trauma, Hainan Medical University, Haikou, China
Search for more papers by this authorAbstract
Rosacea is a complex chronic inflammatory skin disorder with high morbidity. Pyroptosis is known as a regulated inflammatory cell death. While its association with immune response to various inflammatory disorders is well established, little is known about its functional relevance of rosacea. So, we aimed to explore and enrich the pathogenesis involved in pyroptosis-related rosacea aggravations. In this study, we evaluated the pyroptosis-related patterns of rosacea by consensus clustering analysis of 45 ferroptosis-related genes (FRGs), with multiple immune cell infiltration analysis to identify the pyroptosis-mediated immune response in rosacea using GSE65914 dataset. The co–co-work between PRGs and WGCNA-revealed hub genes has established using PPI network. FRG signature was highlighted in rosacea using multi-transcriptomic and experiment analysis. Based on this, three distinct pyroptosis-related rosacea patterns (non/moderate/high) were identified, and the notably enriched pathways have revealed through GO, KEGG and GSEA analysis, especially immune-related pathways. Also, the XCell/MCPcount/ssGSEA/Cibersort underlined the immune-related signalling (NK cells, Monocyte, Neutrophil, Th2 cells, Macrophage), whose hub genes were identified through WGCNA (NOD2, MYD88, STAT1, HSPA4, CXCL8). Finally, we established a pyroptosis-immune co-work during the rosacea aggravations. FRGs may affect the progression of rosacea by regulating the immune cell infiltrations. In all, pyroptosis with its mediated immune cell infiltration is a critical factor during the development of rosacea.
CONFLICT OF INTEREST STATEMENT
The authors declare no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
RNA-sequencing data mentioned in this manuscript can be accessed at GEO database. All of the data have been provided in the Supplementary material.
Supporting Information
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Appendix S1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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