Draft genome sequence and transcriptome analysis of the wine spoilage yeast Dekkera bruxellensis LAMAP2480 provides insights into genetic diversity, metabolism and survival
Corresponding Author
Jorge Valdes
Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile
Millennium Nucleus for Fungal Integrative and Synthetic Biology (MN-FISB), Santiago, Chile
Correspondence: Jorge Valdes, Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile.
Tel.: + 56 223781654;
e-mail: [email protected]
and
Maria Angélica Ganga, Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile.
Tel.: + 56 227184509;
e-mail: [email protected]
Search for more papers by this authorPaz Tapia
Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile
Search for more papers by this authorVictoria Cepeda
Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile
Search for more papers by this authorJavier Varela
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorLiliana Godoy
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorFrancisco A. Cubillos
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Centro de Estudios en Ciencia y Tecnología de los Alimentos (CECTA), Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorEvelyn Silva
Laboratorio de Micología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorClaudio Martinez
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Centro de Estudios en Ciencia y Tecnología de los Alimentos (CECTA), Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorCorresponding Author
Maria Angélica Ganga
Millennium Nucleus for Fungal Integrative and Synthetic Biology (MN-FISB), Santiago, Chile
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Correspondence: Jorge Valdes, Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile.
Tel.: + 56 223781654;
e-mail: [email protected]
and
Maria Angélica Ganga, Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile.
Tel.: + 56 227184509;
e-mail: [email protected]
Search for more papers by this authorCorresponding Author
Jorge Valdes
Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile
Millennium Nucleus for Fungal Integrative and Synthetic Biology (MN-FISB), Santiago, Chile
Correspondence: Jorge Valdes, Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile.
Tel.: + 56 223781654;
e-mail: [email protected]
and
Maria Angélica Ganga, Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile.
Tel.: + 56 227184509;
e-mail: [email protected]
Search for more papers by this authorPaz Tapia
Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile
Search for more papers by this authorVictoria Cepeda
Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile
Search for more papers by this authorJavier Varela
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorLiliana Godoy
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorFrancisco A. Cubillos
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Centro de Estudios en Ciencia y Tecnología de los Alimentos (CECTA), Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorEvelyn Silva
Laboratorio de Micología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorClaudio Martinez
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Centro de Estudios en Ciencia y Tecnología de los Alimentos (CECTA), Universidad de Santiago de Chile, Santiago de Chile, Chile
Search for more papers by this authorCorresponding Author
Maria Angélica Ganga
Millennium Nucleus for Fungal Integrative and Synthetic Biology (MN-FISB), Santiago, Chile
Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile
Correspondence: Jorge Valdes, Bio-Computing and Applied Genetics Division, Fraunhofer Chile Research Foundation, Center for Systems Biotechnology, Santiago, Chile.
Tel.: + 56 223781654;
e-mail: [email protected]
and
Maria Angélica Ganga, Departamento en Ciencia y Tecnología de los Alimentos, Facultad Tecnológica, Universidad de Santiago de Chile, Santiago de Chile, Chile.
Tel.: + 56 227184509;
e-mail: [email protected]
Search for more papers by this authorAbstract
Dekkera bruxellensis is the major contaminant yeast in the wine industry worldwide. Here, we present the draft genome sequence of D. bruxellensis LAMAP2480 isolated from a Chilean wine. Genomic evidence reveals shared and exclusive genes potentially involved in colonization and survival during alcoholic fermentation.
References
- Borneman AR, Zeppel R, Chambers PJ & Curtin CD (2014) Insights into the Dekkera bruxellensis genomic landscape: comparative genomics reveals variations in ploidy and nutrient utilisation potential amongst wine isolates. PLoS Genet 10: e1004161.
- Chatonnet P, Dubordieu D & Boidron J (1995) The influence of Brettanomyces/Dekkera sp. yeasts and lactic acid bacteria on the ethylphenol content of red wines. Am J Enol Vitic 46: 463–468.
- Curtin CD, Borneman AR, Chambers PJ & Pretorius IS (2012) De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast Dekkera bruxellensis AWRI1499. PLoS ONE 7: e33840.
- Godoy L, Garrido D, Martinez C, Saavedra J, Combina M & Ganga MA (2009) Study of the coumarate decarboxylase and vinylphenol reductase activities of Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) isolates. Lett Appl Microbiol 48: 452–457.
- Hellborg L & Piskur J (2009) Complex nature of the genome in a wine-spoilage yeast, Dekkera bruxellensis. Eukaryot Cell 8: 1739–1749.
- Piskur J, Ling Z, Marcet-Houben M et al. (2012) The genome of wine yeast Dekkera bruxellensis provides a tool to explore its food related properties. Int J Food Microbiol 157: 202–209.
- Woolfit M, Rozpedowska E, Piskur J & Wolfe KH (2007) Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensis. Eukaryot Cell 6: 721–733.