Single-Cell RNA Sequencing Reveals the Temporal Diversity and Dynamics of Cardiac Immunity after Myocardial Infarction
Kaiyu Jin
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
Search for more papers by this authorShan Gao
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin Key Laboratory of Translational Research of TCM Prescription and Syndrome, Tianjin, 300193 China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300193 China
Search for more papers by this authorPenghui Yang
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
Search for more papers by this authorRongfang Guo
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
Search for more papers by this authorDan Li
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin Key Laboratory of Translational Research of TCM Prescription and Syndrome, Tianjin, 300193 China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300193 China
Search for more papers by this authorYunsha Zhang
School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193 China
Search for more papers by this authorCorresponding Author
Xiaoyan Lu
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Guanwei Fan
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin Key Laboratory of Translational Research of TCM Prescription and Syndrome, Tianjin, 300193 China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300193 China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin, 301617 China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Xiaohui Fan
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin, 301617 China
Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, 310058 China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorKaiyu Jin
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
Search for more papers by this authorShan Gao
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin Key Laboratory of Translational Research of TCM Prescription and Syndrome, Tianjin, 300193 China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300193 China
Search for more papers by this authorPenghui Yang
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
Search for more papers by this authorRongfang Guo
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
Search for more papers by this authorDan Li
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin Key Laboratory of Translational Research of TCM Prescription and Syndrome, Tianjin, 300193 China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300193 China
Search for more papers by this authorYunsha Zhang
School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193 China
Search for more papers by this authorCorresponding Author
Xiaoyan Lu
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Guanwei Fan
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin Key Laboratory of Translational Research of TCM Prescription and Syndrome, Tianjin, 300193 China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300193 China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin, 301617 China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Xiaohui Fan
Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058 China
State Key Laboratory of Component-Based Chinese Medicine, Tianjin, 301617 China
Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, 310058 China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorAbstract
Myocardial infarction (MI) is strongly associated with the temporal regulation of cardiac immunity. However, a variety of current clinical trials have failed because of the lack of post-MI immunomodulating/anti-inflammatory targets. Single-cell RNA sequencing analysis of the cardiac Cd45+ immune cell at 0, 3, 7, and 14 d after injury in a mouse left anterior descending coronary artery ligation model is performed. Major immune cell populations, distinct subsets, and dynamic changes are identified. Macrophages (Mø) are most abundant, peaking at 3 d after infarction. Mø-5 and Mø-6 are the predominant infiltrated subsets at this time point, with strong expression of inflammatory factors. Further analysis demonstrates that suppressing these sets attenuated pathological MI progression by preventing subsequent leukocyte extravasation and adverse remodeling. Abundant apoptotic neutrophils and a profibrotic macrophage subset on days 7 and 14, respectively, are also detected. These results provide a basis for developing cell type- and time-specific interventions in MI.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are openly available in Gene Expression Omnibus (GEO) at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE163465, reference number 163465.
Supporting Information
| Filename | Description |
|---|---|
| smtd202100752-sup-0001-SuppMat.pdf4.4 MB | Supporting Information |
| smtd202100752-sup-0002-TableS1.xlsx1.1 MB | Supplemental Table 1 |
| smtd202100752-sup-0003-TableS2.xlsx38.6 KB | Supplemental Table 2 |
| smtd202100752-sup-0004-TableS3.xlsx10.7 KB | Supplemental Table 3 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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