Gut-on-a-Chip Reveals Enhanced Peristalsis Reduces Nanoplastic-Induced Inflammation
Junlei Han
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
The Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education, School of Mechanical Engineering, Tianjin University, Tianjin, 300072 China
Search for more papers by this authorYushen Wang
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
Search for more papers by this authorJiemeng Ding
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
Search for more papers by this authorHao Chen
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
Search for more papers by this authorChaoyang Shi
The Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education, School of Mechanical Engineering, Tianjin University, Tianjin, 300072 China
Search for more papers by this authorXinyu Li
Department of Minimally Invasive Comprehensive Treatment of Cancer, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021 China
Search for more papers by this authorZhipeng Xu
Division of Clinical Medicine, School of Medicine and Population Health, University of Sheffield, Medical School, Sheffield, S10 2RX UK
Search for more papers by this authorJun Chen
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
Search for more papers by this authorFeng Kong
Department of Minimally Invasive Comprehensive Treatment of Cancer, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021 China
Search for more papers by this authorCorresponding Author
Li Wang
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
E-mail: [email protected]
Search for more papers by this authorJunlei Han
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
The Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education, School of Mechanical Engineering, Tianjin University, Tianjin, 300072 China
Search for more papers by this authorYushen Wang
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
Search for more papers by this authorJiemeng Ding
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
Search for more papers by this authorHao Chen
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
Search for more papers by this authorChaoyang Shi
The Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education, School of Mechanical Engineering, Tianjin University, Tianjin, 300072 China
Search for more papers by this authorXinyu Li
Department of Minimally Invasive Comprehensive Treatment of Cancer, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021 China
Search for more papers by this authorZhipeng Xu
Division of Clinical Medicine, School of Medicine and Population Health, University of Sheffield, Medical School, Sheffield, S10 2RX UK
Search for more papers by this authorJun Chen
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
Search for more papers by this authorFeng Kong
Department of Minimally Invasive Comprehensive Treatment of Cancer, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021 China
Search for more papers by this authorCorresponding Author
Li Wang
School of Mechanical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353 China
Shandong Institute of Mechanical Design and Research, Jinan, 250353 China
E-mail: [email protected]
Search for more papers by this authorAbstract
Nanoplastics (NPs) pollution is a global issue posing potential threats to human health, particularly the digestive system. NPs may exacerbate intestinal inflammation, increasing the risk of inflammatory bowel disease. However, the impact of intestinal peristalsis on NP-induced inflammation remains unknown. Here, a biomimetic gut-on-a-chip (GOC) with integrated online sensing is presented to investigate NPs' impact on intestinal inflammation and propose enhanced peristalsis as a potential intervention. The GOC simulates intestinal peristalsis through periodic stretching and the optimized sensors dynamically detect inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) over 12 d with low detection limits (0.0095 and 0.0020 pg mL−1). Exposure to NPs led to vacuolization, apoptosis, and loss of tight junction proteins in intestinal cells, with IL-6 and TNF-α secretion peaking at 24 h (1341.55 ± 64.91 and 862.03 ± 66.45 pg mL−1). Notably, increasing periodic strain alleviates inflammatory cytokines secretion induced by NPs. With strain increased from 5% to 6.5%, IL-6 and TNF-α secretion decrease by 2.73-fold and 3.34-fold, respectively. This highlights the protective role of intestinal peristalsis in reducing NP-induced inflammation.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
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| smll202408208-sup-0001-SuppMat.docx21.5 MB | Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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