Volume 19, Issue 6 2370037
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High-Throughput Cellular Heterogeneity Analysis in Cell Migration at the Single-Cell Level (Small 6/2023)

Mengli Zhou

Mengli Zhou

UPMC Hillman Cancer Center, University of Pittsburgh, 5115 Centre Ave, Pittsburgh, PA, 15232 USA

Department of Computational and Systems Biology, University of Pittsburgh, 3420 Forbes Avenue, Pittsburgh, PA, 15260 USA

Xiangya Hospital, Central South University, Changsha, Hunan, 410008 China

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Yushu Ma

Yushu Ma

UPMC Hillman Cancer Center, University of Pittsburgh, 5115 Centre Ave, Pittsburgh, PA, 15232 USA

Department of Computational and Systems Biology, University of Pittsburgh, 3420 Forbes Avenue, Pittsburgh, PA, 15260 USA

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Chun-Cheng Chianga

Chun-Cheng Chianga

UPMC Hillman Cancer Center, University of Pittsburgh, 5115 Centre Ave, Pittsburgh, PA, 15232 USA

Department of Computational and Systems Biology, University of Pittsburgh, 3420 Forbes Avenue, Pittsburgh, PA, 15260 USA

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Edwin C. Rock

Edwin C. Rock

Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, 3700 O'Hara Street, Pittsburgh, PA, 15260 USA

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Kathryn E. Luker

Kathryn E. Luker

Center for Molecular Imaging, Department of Radiology, University of Michigan, 109 Zina Pitcher Place, Ann Arbor, MI, 48109-2200 USA

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Gary D. Luker

Gary D. Luker

Department of Microbiology and Immunology, University of Michigan, 109 Zina Pitcher Place, Ann Arbor, MI, 48109-2200 USA

Center for Molecular Imaging, Department of Radiology, University of Michigan, 109 Zina Pitcher Place, Ann Arbor, MI, 48109-2200 USA

Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel, Blvd, Ann Arbor, MI, 48109-2099 USA

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Yu-Chih Chen

Yu-Chih Chen

UPMC Hillman Cancer Center, University of Pittsburgh, 5115 Centre Ave, Pittsburgh, PA, 15232 USA

Department of Computational and Systems Biology, University of Pittsburgh, 3420 Forbes Avenue, Pittsburgh, PA, 15260 USA

Department of Bioengineering, Swanson School of Engineering, University of Pittsburgh, 3700 O'Hara Street, Pittsburgh, PA, 15260 USA

CMU-Pitt Ph.D. Program in Computational Biology, University of Pittsburgh, 3420 Forbes Avenue, Pittsburgh, PA, 15260 USA

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First published: 08 February 2023

Graphical Abstract

High-Throughput Cellular Heterogeneity

In article number 2206754, Yu-Chih Chen and co-workers present a microfluidic cell migration platform that coordinates robotic liquid handling and computer vision for rapidly quantifying individual cellular motility. The high-throughput single-cell screening experiments reveal that many compounds inhibit migration of most cells while leaving fast-moving subpopulations unaffected. They further investigate dynamic cell movement and discover synergistic drug combinations that overcome the resistance of fast-moving subpopulations.

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