Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore
Qixin Chen
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101 China
Search for more papers by this authorChengzhi Jin
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275 China
Search for more papers by this authorXintian Shao
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101 China
Search for more papers by this authorRuilin Guan
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275 China
Search for more papers by this authorZhiqi Tian
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
Search for more papers by this authorChenran Wang
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
Search for more papers by this authorFei Liu
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101 China
Search for more papers by this authorPeixue Ling
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101 China
Search for more papers by this authorJun-Lin Guan
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
Search for more papers by this authorLiangnian Ji
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275 China
Search for more papers by this authorCorresponding Author
Fengshan Wang
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
E-mail: [email protected], [email protected], [email protected]Search for more papers by this authorCorresponding Author
Hui Chao
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275 China
E-mail: [email protected], [email protected], [email protected]Search for more papers by this authorCorresponding Author
Jiajie Diao
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
E-mail: [email protected], [email protected], [email protected]Search for more papers by this authorQixin Chen
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101 China
Search for more papers by this authorChengzhi Jin
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275 China
Search for more papers by this authorXintian Shao
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101 China
Search for more papers by this authorRuilin Guan
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275 China
Search for more papers by this authorZhiqi Tian
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
Search for more papers by this authorChenran Wang
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
Search for more papers by this authorFei Liu
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101 China
Search for more papers by this authorPeixue Ling
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101 China
Search for more papers by this authorJun-Lin Guan
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
Search for more papers by this authorLiangnian Ji
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275 China
Search for more papers by this authorCorresponding Author
Fengshan Wang
School of Pharmaceutical Sciences, Shandong University, Jinan, 250101 China
E-mail: [email protected], [email protected], [email protected]Search for more papers by this authorCorresponding Author
Hui Chao
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275 China
E-mail: [email protected], [email protected], [email protected]Search for more papers by this authorCorresponding Author
Jiajie Diao
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267 USA
E-mail: [email protected], [email protected], [email protected]Search for more papers by this authorAbstract
Combining luminescent transition metal complex with super-resolution microscopy is an excellent strategy for the long-term visualization of the dynamics of subcellular structures in living cells. However, it remains unclear whether iridium(III) complexes are applicable for a particular type of super-resolution technique, structured illumination microscopy (SIM), to image subcellular structures. Herein, an iridium(III) dye, to track mitochondrial dynamics in living cells under SIM is described. The dye demonstrates excellent specificity and photostability and satisfactory cell permeability. While using SIM to image mitochondria, an ≈80 nm resolution is achieved that allows the clear observation of the structure of mitochondrial cristae. The dye is used to monitor and quantify mitochondrial dynamics relative to lysosomes, including fusion involved in mitophagy, and newly discovered mitochondria–lysosome contact (MLC) under different conditions. The MLC remains intact and fusion vanishes when five receptors, p62, NDP52, OPTN, NBR1, and TAX1BP1, are knocked out, suggesting that these two processes are independent.
Conflict of Interest
The authors declare no conflict of interest.
Supporting Information
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| smll201802166-sup-0001-S1.pdf698.8 KB | Supplementary |
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| smll201802166-sup-0002-S2.avi1.5 MB | Supplementary |
| smll201802166-sup-0003-S3.avi631.8 KB | Supplementary |
| smll201802166-sup-0004-S4.avi224.2 KB | Supplementary |
| smll201802166-sup-0005-S5.avi3 MB | Supplementary |
| smll201802166-sup-0006-S6.avi3.2 MB | Supplementary |
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| smll201802166-sup-0008-S8.avi2.8 MB | Supplementary |
| smll201802166-sup-0009-S9.avi3.2 MB | Supplementary |
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