Single-Molecule FRET Imaging for Enzymatic Reactions at High Ligand Concentrations†
Mitsuhiro Sugawa
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan)
Search for more papers by this authorSo Nishikawa
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan)
Search for more papers by this authorAtsuko Hikikoshi Iwane
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan)
Search for more papers by this authorVasudevanpillai Biju
Nano-Bioanalysis team, Health Technology Research Center National Institute of Advanced Industrial Science and Technology 2217-14 Hayashi-cho, Takamatsu, Kagawa, 761-0395 (Japan)
Search for more papers by this authorCorresponding Author
Toshio Yanagida
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan)
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan).Search for more papers by this authorMitsuhiro Sugawa
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan)
Search for more papers by this authorSo Nishikawa
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan)
Search for more papers by this authorAtsuko Hikikoshi Iwane
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan)
Search for more papers by this authorVasudevanpillai Biju
Nano-Bioanalysis team, Health Technology Research Center National Institute of Advanced Industrial Science and Technology 2217-14 Hayashi-cho, Takamatsu, Kagawa, 761-0395 (Japan)
Search for more papers by this authorCorresponding Author
Toshio Yanagida
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan)
Graduate School of Frontier Biosciences, Osaka University 7F Nanobiology Building, 1-3 Yamadaoka, Suita, Osaka, 565-0871 (Japan).Search for more papers by this authorWe thank K. Oiwa for the kindly gift of Cy3-ATP, T. Komori for helpful discussions, P. Kareginius for carefully reading the manuscript, and colleagues of the Yanagida Lab for suggestions and comments. This work is supported by a Grant-in-Aid for Scientific Research (to S.N.) and the “Yuragi Project” from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Graphical Abstract
Single-molecule Förster resonance energy transfer (FRET) imaging at micromolar concentrations of an analyte is demonstrated. A quantum dot (QD)–dye donor–acceptor pair permits the approach of physiological levels of fluorescently labeled analytes when imaging molecular dynamics. Single-molecule FRET imaging of myosin ATP hydrolysis is performed using QDs attached to myosin as a donor enzyme and Cy3-labeled ATP as an acceptor ligand at micromolar levels (see image).
Supporting Information
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