Polymeric Microneedles for Transdermal Delivery of Human Placental Tissue for the Treatment of Osteoarthritis
Jithu Jerin James
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorSuman Pahal
Institute for Stem Cell Science and Regenerative Medicine, Bengaluru, Karnataka, 560065 India
Institute of Materials Science, Polymer Program, University of Connecticut, Storrs, CT, 06269 USA
Search for more papers by this authorAnbu Jayaraman
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorDamodar Nayak A
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorSridhar Koteshwar Narasimhachar
Cancyte Technologies Pvt Ltd., Sri Shankara Research Centre, Rangadore Memorial Hospital, Shankarapuram, Bengaluru, 560004 India
Search for more papers by this authorSudarson Sundarrajan
Cancyte Technologies Pvt Ltd., Sri Shankara Research Centre, Rangadore Memorial Hospital, Shankarapuram, Bengaluru, 560004 India
Search for more papers by this authorBasavaraj Basappa Veerabhadraiah
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorBharath Srinivasan
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorPraveen Kumar Vemula
Institute for Stem Cell Science and Regenerative Medicine, Bengaluru, Karnataka, 560065 India
Search for more papers by this authorThanh Duc Nguyen
Institute of Materials Science, Polymer Program, University of Connecticut, Storrs, CT, 06269 USA
Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269 USA
Department of Mechanical Engineering, University of Connecticut, Storrs, CT, 06269 USA
Search for more papers by this authorCorresponding Author
Sandhya Kadamboor Veethil
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
E-mail: [email protected]
Search for more papers by this authorJithu Jerin James
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorSuman Pahal
Institute for Stem Cell Science and Regenerative Medicine, Bengaluru, Karnataka, 560065 India
Institute of Materials Science, Polymer Program, University of Connecticut, Storrs, CT, 06269 USA
Search for more papers by this authorAnbu Jayaraman
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorDamodar Nayak A
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorSridhar Koteshwar Narasimhachar
Cancyte Technologies Pvt Ltd., Sri Shankara Research Centre, Rangadore Memorial Hospital, Shankarapuram, Bengaluru, 560004 India
Search for more papers by this authorSudarson Sundarrajan
Cancyte Technologies Pvt Ltd., Sri Shankara Research Centre, Rangadore Memorial Hospital, Shankarapuram, Bengaluru, 560004 India
Search for more papers by this authorBasavaraj Basappa Veerabhadraiah
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorBharath Srinivasan
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
Search for more papers by this authorPraveen Kumar Vemula
Institute for Stem Cell Science and Regenerative Medicine, Bengaluru, Karnataka, 560065 India
Search for more papers by this authorThanh Duc Nguyen
Institute of Materials Science, Polymer Program, University of Connecticut, Storrs, CT, 06269 USA
Department of Biomedical Engineering, University of Connecticut, Storrs, CT, 06269 USA
Department of Mechanical Engineering, University of Connecticut, Storrs, CT, 06269 USA
Search for more papers by this authorCorresponding Author
Sandhya Kadamboor Veethil
Faculty of Pharmacy, M S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, 560054 India
E-mail: [email protected]
Search for more papers by this authorAbstract
Biologics targeting matrix-degrading proteases, cartilage repair, and inflammation are emerging as promising approaches for osteoarthritis (OA) treatment. Recent research highlights biologic-human placental tissue (HPT) as a potential OA therapy due to its biocompatibility, abundant protein biofactors, and ability to reduce cartilage degradation by suppressing protease expression. Microneedles (MNs) are receiving growing attention for enhancing transdermal delivery of biologics as an alternative to conventional subcutaneous injections. The lyophilized human placental extract (LHP) loaded polymeric MNs are fabricated using a micromolding technique for transdermal delivery. Ex vivo release studies reveal that MNs exhibit a gradual and consistent release of LHP, indicating a sustained delivery profile. LHP-MNs are nontoxic and anti-inflammatory in nature against human skin cells and interleukin (IL-1β) induced synovial cells. Furthermore, the in vivo study shows that LHP-MNs substantially improve behavioral parameters in OA rat models and lower serum concentrations of tumor necrosis factor- α (TNF-α) and cartilage oligomeric matrix protein (COMP) biomarkers, thereby alleviating knee and ankle joint injuries. Histopathological analysis indicates that LHP-MNs significantly preserve cartilage integrity. The study results suggest that employing polymeric MNs for transdermal delivery of LHP can be a promising treatment approach for OA, with the added benefit of excellent patient compliance.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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