In Situ Forming Injectable Gelatin-Based Antibacterial Bioadhesives for Preventing Postoperative Leakage and Abdominal Adhesions
Shibo Hu
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorWanglin Duan
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorXianzhen Jin
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorChaowei Li
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorBin Zhu
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorYurong Chen
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorYe Zhu
Department of Ultrasound, The First Medical Centre, Chinese PLA General Hospital, Beijing, 100853 China
Search for more papers by this authorPeihai Cao
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorZepeng Dong
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorLuyao Feng
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorJunhui Yu
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorXuejun Sun
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorZeshaan Yahaya Haji Mahmood
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorCorresponding Author
Yazhong Bu
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
E-mail: [email protected]; [email protected]
Search for more papers by this authorBaoji Du
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorCorresponding Author
Jianbao Zheng
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
E-mail: [email protected]; [email protected]
Search for more papers by this authorShibo Hu
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorWanglin Duan
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorXianzhen Jin
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorChaowei Li
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorBin Zhu
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorYurong Chen
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorYe Zhu
Department of Ultrasound, The First Medical Centre, Chinese PLA General Hospital, Beijing, 100853 China
Search for more papers by this authorPeihai Cao
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorZepeng Dong
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorLuyao Feng
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorJunhui Yu
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorXuejun Sun
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorZeshaan Yahaya Haji Mahmood
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorCorresponding Author
Yazhong Bu
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
E-mail: [email protected]; [email protected]
Search for more papers by this authorBaoji Du
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
Search for more papers by this authorCorresponding Author
Jianbao Zheng
Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 China
E-mail: [email protected]; [email protected]
Search for more papers by this authorAbstract
Postoperative anastomotic leakage (AL) and abdominal adhesions are two major complications after intestinal surgery, with an incidence of 2–25% for AL and 93% for adhesion. Until now, there is no method addressing AL and abdominal adhesions simultaneously. In this work, Bi-PEG–succinimidyl succinate (PEG-NHS), amino-gelatin (Agel) is combined with cefoperazone-sulbactam (SCF) to prepare a multifunctional bioadhesive (SCF/SEAgel) for the postoperative leakage and adhesion prevention. SCF/SEAgel possesses a great sealing capability for tissue, with a bursting pressure of 54 kPa. The loaded SCF endows the systems with good antibacterial properties. The in vivo antiadhesion experiments show that SCF/SEAgel possesses better anti-adhesion properties than the commercially used sodium hyaluronate gel. In the cecum leakage model, the SCF/SEAgel effectively seals the leakage with a survival rate of 100%, superior to commercial products (Sainaoning). Meanwhile, it significantly reduces tissue adhesion. Finally, the laparoscopic surgery with dogs shows that the SCF/SEAgel can be injected through minimally invasive surgery, demonstrating its ease of use. Combined with its great biocompatibility, SCF/SEAgel is very promising in intestinal surgery.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| mabi202400372-sup-0001-SuppMat.docx6.4 MB | Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
References
- 1S. A. Rojas-Machado, M. Romero, A. Arroyo, A. Rojas-Machado, J. López, R. Calpena, Int. J. Surg. 2016, 27, 92.
- 2C. S. McArdle, D. C. McMillan, D. J. Hole, Br. J. Surg. 2005, 92, 1150.
- 3N. C. Buchs, P. Gervaz, M. Secic, P. Bucher, B. Mugnier-Konrad, P. Morel, Int. J. Colorectal Dis. 2008, 23, 265.
- 4E. C. Zarnescu, N. O. Zarnescu, R. Costea, Diagnostics 2021, 11, 2382.
- 5J. T. Ross, M. A. Matthay, H. W. Harris, BMJ 2018, 361, k1407.
- 6A. Alves, Y. Panis, M. Pocard, J. M. Regimbeau, P. Valleur, J. Am. Coll. Surg. 1999, 189, 554.
- 7A. A. Khan, J. M. Wheeler, C. Cunningham, B. George, M. Kettlewell, N. J. Mortensen, Colorectal Dis. 2008, 10, 587.
- 8O. Hallböök, R. Sjödahl, Br. J. Surg. 1996, 83, 60.
- 9A. Nesbakken, K. Nygaard, O. C. Lunde, Br. J. Surg. 2001, 88, 400.
- 10F. E. Turrentine, C. E. Denlinger, V. B. Simpson, R. A. Garwood, S. Guerlain, A. Agrawal, C. M. Friel, D. J. LaPar, G. J. Stukenborg, R. S. Jones, J. Am. Coll. Surg. 2015, 220, 195.
- 11E. Dorkhani, Y. Noorafkan, R. A. Asbagh, M. Okhovat, A. Heirani-Tabasi, S. M. Ahmadi Tafti, Front. Surg. 2022, 9, 1018590.
- 12A. Bal-Ozturk, B. Cecen, M. Avci-Adali, S. N. Topkaya, E. Alarcin, G. Yasayan, Y. C. Ethan, B. Bulkurcuoglu, A. Akpek, H. Avci, K. Shi, S. R. Shin, S. Hassan, Nano Today 2021, 36, 101049.
- 13L. Ge, S. Chen, Polymers 2020, 12, 939.
- 14S. B. Park, Y. J. Yum, J. M. Cha, Medicine 2021, 100, e28075.
- 15S. Panda, M. P. Connolly, M. G. Ramirez, J. Beltrán de Heredia, Risk Manag. Healthcare Policy 2020, 13, 5.
- 16G. Nandakumar, B. G. Richards, K. Trencheva, G. Dakin, Surg. Obes. Relat. Dis. 2010, 6, 498.
- 17Z. Wu, K. A. Vakalopoulos, G. S. Boersema, L. F. Kroese, K. H. Lam, P. H. van der Horst, I. M. Mulder, Y. M. Bastiaansen-Jenniskens, G. J. Kleinrensink, J. Jeekel, J. F. Lange, Int. J. Colorectal Dis. 2014, 29, 1507.
- 18G. Pascual, S. Sotomayor, M. Rodríguez, B. Pérez-Köhler, A. Kühnhardt, M. Fernández-Gutiérrez, J. San Román, J. M. Bellón, PLoS One 2016, 11, e0157920.
- 19A. Luk, T. E. David, J. Butany, J. Clin. Pathol. 2012, 65, 1008.
- 20P. Wang, Y. Zhu, L. Feng, Y. Wang, Y. Bu, ACS Appl. Mater. Interfaces 2022, 14, 373.
- 21J. Tang, Z. Xiang, M. T. Bernards, S. Chen, Acta Biomater. 2020, 116, 84.
- 22R. M. Leclercq, K. W. Van Barneveld, M. H. Schreinemacher, R. Assies, M. Twellaar, N. D. Bouvy, J. W. Muris, Eur. J. Gen. Pract. 2015, 21, 176.
- 23M. P. Diamond, S. D. Wexner, G. S. diZereg, M. Korell, O. Zmora, H. Van Goor, M. Kamar, Surg. Innov. 2010, 17, 183.
- 24V. Sikirica, B. Bapat, S. D. Candrilli, K. L. Davis, M. Wilson, A. Johns, BMC Surg. 2011, 11, 13.
- 25J. H. Ryu, H. J. Kim, K. Kim, G. Yoon, Y. Wang, G.-S. Choi, H. Lee, J. S. Park, Adv. Funct. Mater. 2019, 29, 1900495.
- 26Y. Bu, H. Shen, F. Yang, Y. Yang, X. Wang, D. Wu, ACS Appl. Mater. Interfaces 2017, 9, 2205.
- 27Y. Uno, Clin. Exp. Gastroenterol. 2018, 11, 153.
- 28Q. Mao, Z. Huang, Y. Zhang, Q. Chen, K. Jiang, Y. Hong, H. Ouyang, Y. Liang, Adv. Healthcare Mater. 2023, 12, e2301379.
- 29J. Hammond, S. Lim, Y. Wan, X. Gao, A. Patkar, J. Gastrointest. Surg. 2014, 18, 1176.
- 30A. H. Araten, R. S. Brooks, S. D. W. Choi, L. L. Esguerra, D. Savchyn, E. J. Wu, G. Leon, K. J. Sniezek, M. P. Brynildsen, J. Antibiot. 2024, 77, 135.
- 31S. K. Nair, I. K. Bhat, A. L. Aurora, Arch. Surg. 1974, 108, 849.
- 32A. R. Novotny, D. Reim, V. Assfalg, F. Altmayr, H. M. Friess, K. Emmanuel, B. Holzmann, Immunobiology 2012, 217, 616.
- 33P. X. Liew, P. Kubes, Physiol. Rev. 2019, 99, 1223.
- 34M. R. Pinsky, J. L. Vincent, J. Deviere, M. Alegre, R. J. Kahn, E. Dupont, Chest 1993, 103, 565.
- 35R. Song, J. Kim, D. Yu, C. Park, J. Park, Vet. Immunol. Immunopathol. 2012, 146, 143.
- 36J. Menz, L. Hundt, T. Schulze, K. Schmoeckel, P. Menges, G. Domanska, Sci. Rep. 2021, 11, 16175.
- 37V. Karamouzos, E. J. Giamarellos-Bourboulis, D. Velissaris, T. Gkavogianni, C. Gogos, Infect. Dis. 2021, 53, 764.
- 38H. S. Himal, Surg. Endosc. Other Interven. Tech. 2002, 16, 1647.
- 39A. G. Harrell, B. T. Heniford, Am. J. Surg. 2005, 190, 239.
