A sensitive electrochemical DNA sensor based on reduced graphene oxide modified electrode
Binxiang Xu
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorYuping Hu
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorQingxia Shu
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorMei Wang
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorZhiyang Chen
Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorWei Wei
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorJinmei Wen
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorRui Li
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorFusheng Liao
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorCorresponding Author
Lin Cheng
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Correspondence
Lin Cheng and Hao Fan, Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, JiangXi 330004, China.
Email: [email protected] (L. C.) and [email protected] (H. F.)
Search for more papers by this authorCorresponding Author
Hao Fan
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Correspondence
Lin Cheng and Hao Fan, Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, JiangXi 330004, China.
Email: [email protected] (L. C.) and [email protected] (H. F.)
Search for more papers by this authorBinxiang Xu
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorYuping Hu
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorQingxia Shu
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorMei Wang
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorZhiyang Chen
Department of Postgraduate, Jiangxi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorWei Wei
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorJinmei Wen
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorRui Li
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorFusheng Liao
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Search for more papers by this authorCorresponding Author
Lin Cheng
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Correspondence
Lin Cheng and Hao Fan, Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, JiangXi 330004, China.
Email: [email protected] (L. C.) and [email protected] (H. F.)
Search for more papers by this authorCorresponding Author
Hao Fan
Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, China
Correspondence
Lin Cheng and Hao Fan, Department of Pharmacy, JiangXi University of Traditional Chinese Medicine, Nanchang, JiangXi 330004, China.
Email: [email protected] (L. C.) and [email protected] (H. F.)
Search for more papers by this authorBinxiang Xu and Yuping Hu contributed equally to this study.
Funding information: Natural Science Foundation of Jiangxi Province, Grant/Award Numbers: 20192BAB205103, 20202BABL206152; National College Student Innovation and Entrepreneurship Training Program Support Project, Grant/Award Number: 202110412016; National Natural Science Foundation of China, Grant/Award Numbers: 80000000, 81860682, 81872968; Science and technology project of the Education Department of JiangXi Province, Grant/Award Number: GJJ180654
Abstract
In this work, an electrochemical sensing platform, analogous to a DNA walker, was explored with exonuclease III (EXO III) as a driving force, tetraferrocene as a signal marker, and reduced graphene oxide (RGO) electrode as two-dimensional (2D) orbit to realize ultrasensitive detection of target DNA. We designed a single hairpin DNA probe with 3′-end modified tetraferrocene, immobilized on the electrode surface by π-π stacking between the 5′ terminus and reduced graphene oxide. In addition, EXO III digested the double-stranded DNA with a blunt 3′ terminus formed by the target DNA and the probe DNA, thus degrading the 3′ end of probe DNA, and resulting in the away movement of tetraferrocene from the electrode surface, decreasing the electrochemical signal. The proposed electrochemical sensor was very simple and stable with acceptable reproducibility, excellent DNA detection performance, and a detection limit as low as 0.3 pM.
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Supporting Information
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REFERENCES
- 1S. Hardikar, Z. Ying, Y. Zeng, H. Zhao, B. Liu, N. Veland, K. McBride, X. Cheng, T. Chen, Protein Cell 2020, 11, 214.
- 2G. F. Codner, V. Erbs, J. Loeffler, L. Chessum, A. Caulder, N. Jullien, S. Wells, M.-C. Birling, L. Teboul, Methods 2021, 191, 59.
- 3Y. Nie, X. Zhang, Q. Zhang, Z. Liang, Q. Ma, X. Su, Biosens. Bioelectron. 2020, 160, 112117.
- 4R. Cai, S. Zhang, L. Chen, M. Li, Y. Zhang, N. Zhou, ACS Appl. Mater. Interf. 2021, 13, 4905.
- 5H. Liu, J. Wang, H. Jin, M. Wei, W. Ren, Y. Zhang, L. Wu, B. He, Sens. Actuat. B: Chem. 2021, 329, 129215.
- 6Y. Qi, Y. Chen, J. He, F. Xiu, Microchem. J. 2020, 159, 105546.
- 7R. Zou, Y. Ma, C. Li, F. Zhang, C. Chen, C. Cai, Microchem. J. 2020, 156, 104764.
- 8G. Chen, R. Chen, S. Ding, M. Li, J. Wang, J. Zou, F. Du, J. Dong, X. Cui, X. Huang, Y. Deng, Z. Tang, Analyst 2020, 145, 440.
- 9W. Jaroenram, J. Kampeera, N. Arunrut, C. Karuwan, W. Kiatpathomchai, J Pharmaceut. Biomed. Anal. 2020, 186, 113333.
- 10X. Liu, X. Zhou, X. Xia, H. Xiang, Analytica Chimica Acta 2020, 1096, 159.
- 11O. L. Bodulev, K. M. Burkin, E. E. Efremov, I. Y. Sakharov, Anal. Bioanal. Chem. 2020, 412, 5105.
- 12Y. Liao, J. Gao, Y. Zhang, Y. Zhou, W. Xu, Sens. Actuators B Chem. 2020, 322, 128566.
- 13N. Liao, M. C. Pan, L. Wang, F. Yang, Y. Zhuo, Anal. Chem. 2021, 93, 4051.
- 14L. Wang, P. Liu, Z. Liu, K. Zhao, J. J. Zhu, ACS Sens. 2020, 5, 3584.
- 15M. Chen, R. Duan, S. Xu, Z. Duan, Q. Yuan, F. Xia, F. Huang, Anal. Chem. 2018, 93, 16264.
- 16H. Lee, D. M. Kim, D. E. Kim, Analyst 2021, 145, 8002.
- 17L. Wang, H. Zeng, X. Yang, C. Chen, S. Ou, Microchimica Acta 2021, 188, 1.
- 18D. Li, Y. Xu, L. Fan, B. Shen, X. Ding, R. Yuan, X. Li, W. Chen, Biosens. Bioelectron. 2020, 148, 11826.
- 19J. Valero, M. Skugor, Chemphyschem 1971, 2020, 21.
- 20B. Mxa, B. Dt, Analytica Chimica Acta. 2021, 1171, 338523.
- 21P. W. K. Rothemund, Nature 2006, 440, 297.
- 22Q. Feng, X. Zhao, Y. Guo, M. Liu, P. Wang, Biosens. Bioelectron. 2018, 108, 97.
- 23E. Mauri, A. Salvati, A. Cataldo, P. Mozetic, F. Basoli, F. Abbruzzese, M. Trombetta, S. Bellucci, A. Rainer, Mater. Sci. Eng. C-Mater. Biol. Appl. 2021, 118, 111353.
- 24M. Coros, S. Pruneanu, R.-I. Stefan-van Staden, J. Electrochem. Soc. 2019, 167, 37528.
- 25M. Oprea, S. I. Voicu, Materials 2020, 13, 5347.
- 26G. Cao, J. Yan, X. Ning, Q. Zhang, Q. Wu, L. Bi, Y. Zhang, Y. Han, J. Guo, Colloid. Surf. B-Biointerf. 2021, 200, 111588.
- 27A. Pathak, M. Meena, S. Tripathi, Phys. Solid State 2021, 63, 453.
- 28H. Rao, Z. Xue, X. Wang, G. Zhao, H. Hou, H. Wang, Prog. Chem. 2016, 28, 337.
- 29G. Mo, X. He, C. Zhou, D. Ya, J. Feng, C. Yu, B. Deng, Biosens. Bioelectron. 2019, 126, 558.
- 30X. Zhang, Y. Jiang, M. Zhu, Y. Xu, Z. Guo, J. Shi, E. Han, X. Zou, D. Wang, Chem. Eng. J. 2020, 383, 123182.
- 31Y. Tingting, Z. Longyi, J. Huangxian, L. Jianping, Anal. Chem. 2018, 90, 14493.
- 32F. Otero, K. Shortall, U. Salaj-Kosla, S. A. M. Tofail, E. Magner, Electrochimica Acta 2021, 388, 138642.
- 33L. Tian, J. Qi, X. Ma, X. Wang, C. Yao, W. Song, Y. Wang, Biosens. Bioelectron. 2018, 122, 43.
- 34B. Zhang, P. Tian, H. Zhu, L. Xie, B. He, J. Hazard. Mater. 2021, 416, 125831.
- 35N. Wang, Y. J. Jiang, X. Zhang, H. R. Lin, C. Z. Huang, Analyst 2021, 146, 1675.
- 36A. Rabti, C. C. Mayorga-Martinez, L. Baptista-Pires, N. Raouafi, A. Merkoci, Analytica Chimica Acta 2016, 926, 28.
- 37J. Kudr, L. Richtera, L. Nejdl, K. Xhaxhiu, R. Kizek, Materials 2016, 9, 1.
- 38H. Imran, P. N. Manikandan, V. Dharuman, J. Electroanal. Chem. 2019, 835, 10.
- 39S.-H. Wu, B. Zhang, F.-F. Wang, Z.-Z. Mi, J.-J. Sun, Biosens. Bioelectron. 2018, 104, 145.
- 40Z. Chen, S. Chengjun, L. Zewei, L. Kunping, Y. Xijian, Z. Haimin, L. Yongxin, D. Yixiang, Sens. Actuat. B Chem. 2017, 254, 956.
- 41F. Zhang, L. Xiang, X. Xiao, X. Chen, C. Chen, C. Cai, Analyst 2020, 145, 206.
- 42N. Li, Y. Sun, F. Wang, C. Huang, C. Fu, L. Zhang, Y. Liu, S. Ge, J. Yu, Sens. Actuat. B: Chem. 2021, 345, 130386.
- 43Y. Ning, X. Wang, J. Hu, L. Li, R. Xiao, F. Lu, Microchimica Acta 2021, 188, 1.
- 44Y. Sun, C. Huang, X. Sun, Q. Wang, P. Zhao, S. Ge, J. Yu, Microchimica Acta 2021, 188, 1.
