Multi-color two-laser super-resolution structured illumination microscopy for the visualization of multi-organelle in living cells
Corresponding Author
Xuejuan Hu
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Correspondence
Xuejuan Hu, Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, Guang-dong, China.
Email: [email protected]
Yujie Huang, College of Pharmacy, Shenzhen, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, China.
Email: [email protected]
Lihu Wang, College of Physics Science and Technology, Guangxi Normal University, 15 Yucai Road and 0773-3696880, 541004 Guilin City, China.
Email: [email protected]
Search for more papers by this authorYadan Tan
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorCorresponding Author
Yujie Huang
College of Pharmacy, Shenzhen Technology University, Shenzhen, Guangdong, China
Correspondence
Xuejuan Hu, Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, Guang-dong, China.
Email: [email protected]
Yujie Huang, College of Pharmacy, Shenzhen, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, China.
Email: [email protected]
Lihu Wang, College of Physics Science and Technology, Guangxi Normal University, 15 Yucai Road and 0773-3696880, 541004 Guilin City, China.
Email: [email protected]
Search for more papers by this authorJianze Ye
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorYifei Liang
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorXiaokun Yang
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorHengliang Wang
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorZihao Cheng
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorCorresponding Author
Lihu Wang
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Correspondence
Xuejuan Hu, Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, Guang-dong, China.
Email: [email protected]
Yujie Huang, College of Pharmacy, Shenzhen, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, China.
Email: [email protected]
Lihu Wang, College of Physics Science and Technology, Guangxi Normal University, 15 Yucai Road and 0773-3696880, 541004 Guilin City, China.
Email: [email protected]
Search for more papers by this authorShiqian Liu
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorMinfei Li
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorZhengdi He
College of Health and Environmental Engineering, Shenzhen Technology University, Shenzhen, Guangdong, China
Search for more papers by this authorQianding Gao
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorJingli Zhong
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorCorresponding Author
Xuejuan Hu
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Correspondence
Xuejuan Hu, Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, Guang-dong, China.
Email: [email protected]
Yujie Huang, College of Pharmacy, Shenzhen, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, China.
Email: [email protected]
Lihu Wang, College of Physics Science and Technology, Guangxi Normal University, 15 Yucai Road and 0773-3696880, 541004 Guilin City, China.
Email: [email protected]
Search for more papers by this authorYadan Tan
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorCorresponding Author
Yujie Huang
College of Pharmacy, Shenzhen Technology University, Shenzhen, Guangdong, China
Correspondence
Xuejuan Hu, Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, Guang-dong, China.
Email: [email protected]
Yujie Huang, College of Pharmacy, Shenzhen, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, China.
Email: [email protected]
Lihu Wang, College of Physics Science and Technology, Guangxi Normal University, 15 Yucai Road and 0773-3696880, 541004 Guilin City, China.
Email: [email protected]
Search for more papers by this authorJianze Ye
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorYifei Liang
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorXiaokun Yang
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorHengliang Wang
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorZihao Cheng
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorCorresponding Author
Lihu Wang
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Correspondence
Xuejuan Hu, Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, Guang-dong, China.
Email: [email protected]
Yujie Huang, College of Pharmacy, Shenzhen, Shenzhen Technology University, 3002 Lantian Road and 0755-23256054, 518118 Shenzhen City, China.
Email: [email protected]
Lihu Wang, College of Physics Science and Technology, Guangxi Normal University, 15 Yucai Road and 0773-3696880, 541004 Guilin City, China.
Email: [email protected]
Search for more papers by this authorShiqian Liu
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorMinfei Li
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorZhengdi He
College of Health and Environmental Engineering, Shenzhen Technology University, Shenzhen, Guangdong, China
Search for more papers by this authorQianding Gao
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorJingli Zhong
College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China
Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen, Guang-dong, China
Search for more papers by this authorAbstract
In this study, we introduced a novel dual-laser multi-color imaging system. Integrated with a multi-channel filter wheel, this system compared three spectral decontamination algorithms (nonnegative matrix factorization [NMF], RCAN, and PICASSO) showcasing its efficacy in achieving four-color imaging with only two laser sources. Combined with a reliable image reconstruction algorithm, the spatial resolution of four channels super-resolution four-color images reached 130, 125, 133, and 132 nm, respectively. Lipid droplets, mitochondria, lysosomes, and nuclei from the mouse hepatocytes (AML12), human neuroblastoma cells (SH-SY5Y), mouse hippocampal neuronal cells (HT-22), and immortalized murine bone marrow-derived macrophages were imaged. At the same time, the chromatin condensation, nuclear contraction, DNA fragmentation, apoptotic body formation, as well as the fusion of Mito and Lyso involved in mitochondrial autophagy were observed in HT-22 and SH-SY5Y cells suffering oxidative stress. Our multi-color SIM imaging system establishes a powerful platform for dynamic organelle studies and other high-resolution investigations in live cells.
CONFLICT OF INTEREST STATEMENT
The authors declare no financial or commercial conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| jbio202400154-sup-0001-supinfo.docxWord 2007 document , 1.4 MB | Data S1. Supporting Information. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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