Review Article

Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues

Paolo Bianchini

LAMBS, MicroScoBiO Research Center and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy

IFOM, FIRC Institute for Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy

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Alberto Diaspro,

Alberto Diaspro

[email protected]

LAMBS, MicroScoBiO Research Center and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy

IFOM, FIRC Institute for Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy

Department of Neuroscience and Brain Technology, IIT Italian Institute of Technology, Morego, Italy

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Paolo Bianchini,

Paolo Bianchini

LAMBS, MicroScoBiO Research Center and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy

IFOM, FIRC Institute for Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy

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Alberto Diaspro,

Alberto Diaspro

[email protected]

LAMBS, MicroScoBiO Research Center and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy

IFOM, FIRC Institute for Molecular Oncology Foundation, Via Adamello 16, 20139 Milan, Italy

Department of Neuroscience and Brain Technology, IIT Italian Institute of Technology, Morego, Italy

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First published: 05 December 2008

https://doi.org/10.1002/jbio.200810060

Citations: 61

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Abstract

In this work we aim to show how it is possible to exploit the second harmonic generation (SHG) signal for producing multimodal microscopic images of biological tissues. SHG microscopy constitutes an important tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architectures. The physical origins of SHG within these tissues are addressed and reported in a comprehensive image gallery. Although SHG is a coherent process, the multiple scattering of tissue samples determines the ability to acquire signal in both backward and forward direction. We discuss here some key elements related to the backward and forward SHG signal in terms of acquisition architecture and related microscopic imaging. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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Volume1, Issue6

Special Issue:Multiphoton Imaging and FLIM

December 2008

Pages 443-450

Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues

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Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues

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