In vitro and in vivo imaging of xenobiotic transport in human skin and in the rat liver†
Corresponding Author
Michael S. Roberts
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Phone: +61732402546 Fax: +61732405806Search for more papers by this authorMatthew J. Roberts
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorThomas A. Robertson
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorWashington Sanchez
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorCamilla Thörling
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorYuhong Zou
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorXin Zhao
Physics, Macquarie University, Sydney, NSW 2109, Australia
Search for more papers by this authorWolfgang Becker
Becker & Hickl GmbH, Nahmitzer Damm 30, 12277 Berlin, Germany
Search for more papers by this authorAndrei V. Zvyagin
Physics, Macquarie University, Sydney, NSW 2109, Australia
Search for more papers by this authorCorresponding Author
Michael S. Roberts
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Phone: +61732402546 Fax: +61732405806Search for more papers by this authorMatthew J. Roberts
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorThomas A. Robertson
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorWashington Sanchez
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorCamilla Thörling
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorYuhong Zou
Therapeutics Research Unit, Department of Medicine, University of Queensland, Princess Alexandra Hospital, Woolloongabba, QLD 4102, Australia
Search for more papers by this authorXin Zhao
Physics, Macquarie University, Sydney, NSW 2109, Australia
Search for more papers by this authorWolfgang Becker
Becker & Hickl GmbH, Nahmitzer Damm 30, 12277 Berlin, Germany
Search for more papers by this authorAndrei V. Zvyagin
Physics, Macquarie University, Sydney, NSW 2109, Australia
Search for more papers by this authorAbstracted from a lecture presented at FLIM2008, Saarbrucken, Germany, June 2008
Abstract
Multiphoton tomography was used to examine xenobiotic transport in vivo. We used the photochemical properties of zinc oxide and fluorescein and multiphoton tomography to study their transport in the skin and in the rat liver in vivo. Zinc oxide nanoparticles were visualised in human skin using the photoluminescence properties of zinc oxide and either a selective emission wavelength band pass filter or a filter with fluorescence lifetime imaging (FLIM). Zinc oxide nanoparticles (30 nm) did not penetrate into human skin in vitro and in vivo and this was validated by scanning electron microscopy with X-ray photoelectron spectroscopy. Fluorescein was measured in the liver using FLIM. Fluorescein is rapidly extracted from the blood into the liver cells and then transported into the bile. It is suggested that multiphoton tomography may be of particular use in defining in vivo 4D (in both space and time) pharmacokinetics. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
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