Photonics Research Institute, National Institute of Advanced Industrial Science and Technology AIST, 1-8-31, Midorigaoka, Ikeda, Osaka 563-8577, Japan

Search for more papers by this author

Karsten König,

Corresponding Author

Karsten König

[email protected]

Saarland University, Faculty of Mechatronics and Physics, Campus, 66123 Saarbrücken, Germany

JenLab GmbH, Schillerstraße 1, 07745 Jena, Germany

Phone: +49 6813023451, Fax: +49 6813023090Search for more papers by this author

Aisada Uchugonova,

Aisada Uchugonova

Fraunhofer Institute for Biomedical Technology IBMT, Ensheimer Straße 48, 66386 St. Ingbert, Germany

Saarland University, Faculty of Mechatronics and Physics, Campus, 66123 Saarbrücken, Germany

Search for more papers by this author

Andreas Isemann,

Andreas Isemann

FEMTOLASERS Produktions GmbH, Fernkorngasse 10, 1100 Vienna, Austria

Search for more papers by this author

Erwin Gorjup,

Erwin Gorjup

Fraunhofer Institute for Biomedical Technology IBMT, Ensheimer Straße 48, 66386 St. Ingbert, Germany

Search for more papers by this author

Gabriel Tempea,

Gabriel Tempea

FEMTOLASERS Produktions GmbH, Fernkorngasse 10, 1100 Vienna, Austria

Search for more papers by this author

Rainer Bückle,

Rainer Bückle

JenLab GmbH, Schillerstraße 1, 07745 Jena, Germany

Search for more papers by this author

Wataru Watanabe,

Wataru Watanabe

Photonics Research Institute, National Institute of Advanced Industrial Science and Technology AIST, 1-8-31, Midorigaoka, Ikeda, Osaka 563-8577, Japan

Search for more papers by this author

Karsten König,

Corresponding Author

Karsten König

[email protected]

Saarland University, Faculty of Mechatronics and Physics, Campus, 66123 Saarbrücken, Germany

JenLab GmbH, Schillerstraße 1, 07745 Jena, Germany

Phone: +49 6813023451, Fax: +49 6813023090Search for more papers by this author

First published: 05 December 2008

https://doi.org/10.1002/jbio.200810047

Citations: 21

About

PDF

Tools

Share a link

Email
Wechat
Bluesky

Abstract

Novel ultracompact multiphoton sub-20 femtosecond near infrared 85 MHz laser scanning microscopes and conventional 250 fs laser microscopes have been used to perform high spatial resolution two-photon imaging of stem cell clusters as well as selective intracellular nanoprocessing and knock out of living single stem cells within an 3D microenvironment without any collateral damage. Also lethal cell exposure of large parts of cell clusters was successfully probed while maintaining single cells of interest alive. The mean power could be kept in the milliwatt range for 3D nanoprocessing and even in the microwatt range for two-photon imaging. Ultracompact low power sub-20 fs laser systems may become interesting tools for optical nanobiotechnology such as optical cleaning of stem cell clusters as well as optical transfection. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

References

[1] W. Denk, J. Strickler, and W. Webb, Sciences 248 (4951), 73–76 (1990).
Google Scholar
[2] W. Watanabe, S. Matsunaga, T. Shimada, T. Higashi, K. Fukui, and K. Itoh, Med. Laser Appl. 20, 185–191 (2005).
10.1016/j.mla.2005.07.004
Google Scholar
[3] A. Heisterkamp, I. Z. Maxwell, E. Mazur, J. M. Underwood, J. A. Nickerson, S. Kumar, and D. E. Ingber, Opt. Express 13(10), 3690–3696 (2005).
10.1364/OPEX.13.003690
CAS PubMed Web of Science® Google Scholar
[4] T. Shimada, W. Watanabe, S. Matsunaga, T. Higashi, H. Ishii, K. Fukui, K. Isobe, and K. Itoh, Opt. Express 13(24), 9869–9880 (2005).
10.1364/OPEX.13.009869
PubMed Web of Science® Google Scholar
[5] L. Sacconi, I. Tolic-Norrelykke, R. Antolini, and F. S. Pavone, J. Biomed. Opt. 10, 14002 (2005).
10.1117/1.1854675
PubMed Web of Science® Google Scholar
[6] K. König, I. Riemann, and W. Fritzsche, Opt. Lett. 268, 19–821 (2001).
Google Scholar
[7] K. König, O. Krauss, and I. Riemann, Opt. Express 10(3), 171–176 (2002).
10.1364/OE.10.000171
PubMed Web of Science® Google Scholar
[8] U. K. Tirpalur and K. König, Nature 418, 4295–4298 (2002).
Google Scholar
[9] J. Baumgart, W. Bintig, A. Ngezahayo, S. Willenbrock, H. M. Escobar, W. Ertmer, H. Lubatschovski, and A. Heisterkamp, Opt. Express 16(5), 3021–3031 (2008).
10.1364/OE.16.003021
PubMed Web of Science® Google Scholar
[10] V. Kohli, A. Elezzabi, and J. P. Acker, Lasers Surg. Med. 37, 227–230 (2005).
10.1002/lsm.20220
CAS PubMed Web of Science® Google Scholar
[11] V. Kohli and A. Y. Elezzabi, BMC Biotechnol. 2008, 8: 7.
Google Scholar
[12] A. Uchugonova, K. König, R. Bueckle, A. Isemann, and G. Tempea, Opt. Express 16(13), 9357–9364 (2008).
10.1364/OE.16.009357
CAS PubMed Web of Science® Google Scholar
[13] R. Jaenisch and R. Young, Cell 132, 567–582 (2008).
10.1016/j.cell.2008.01.015
CAS PubMed Web of Science® Google Scholar
[14] M. Körbling, Z. Estrov, and R. Champlin, Bone Marrow Transplant. 32, 23–24 (2003).
10.1038/sj.bmt.1703939
CAS PubMed Web of Science® Google Scholar
[15] L. Jackson, D. R. Jones, P. Scotting, and V. Sottile, J. Postgrad. Med. 53(2), 121–127 (2007).
10.4103/0022-3859.32215
CAS PubMed Web of Science® Google Scholar
[16] C. M. Rice and N. J. Scolding, J. Neurosci. 265, 59–62 (2008).
PubMed Web of Science® Google Scholar
[17] W. Lillge, 21st Century Science &>; Technology magazine, Winter 2001–2002.
Google Scholar
[18] S. Gerecht-Nir and J. Itskovitz-Eldor, Transpl. Immunol. 12, 203–209 (2004).
10.1016/j.trim.2003.12.013
CAS PubMed Web of Science® Google Scholar
[19] D. Rubio, J. Garcia-Castro, M. C. Martin, R. Fuente, J. C. Cigudosa, A. C. Lloyd, and A. Bernad, Cancer Res. 65(8), 3035–3039 (2005).
10.1158/0008-5472.CAN-04-4194
CAS PubMed Web of Science® Google Scholar
[20] C. Kruse, J. Kajahn, A. E. Petschnik, A. Maass, E. Klink, D. N. Rapoport, and T. Wedel, Ann. of Anatomy 18(8), 503–517 (2006).
10.1016/j.aanat.2006.07.012
Web of Science® Google Scholar
[21] C. Kruse, M. Birth, J. Rohwedel, K. Assmuth, A. Goepel, and T. Wedel, Appl. Phys. A 79, 1617–1624 (2004).
10.1007/s00339-004-2816-6
CAS Web of Science® Google Scholar
[22] F. Thevenod, J. M. Friedmann, A. D. Katsen, and I. A. Hauser, J. Biol. Chem. 275(3), 1887–1896 (2000).
10.1074/jbc.275.3.1887
PubMed Web of Science® Google Scholar
[23] A. Uchugonova and K. König, J. Biomed. Opt., in Press.
Google Scholar