Orostachys japonicus exerts antipancreatic cancer activity through induction of apoptosis and cell cycle arrest in PANC-1 cells
Ji Hyun Kim,
Gi Suk Nam,
Sung Hyun Kim,
Deok Seon Ryu,
Dong Seok Lee,
Ji Hyun Kim
Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Korea
Search for more papers by this authorGi Suk Nam
Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Korea
Search for more papers by this authorSung Hyun Kim
Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Korea
Search for more papers by this authorDeok Seon Ryu
Department of Biomedical Laboratory Science, Soonchunhyang University, Asan, Korea
Search for more papers by this authorCorresponding Author
Dong Seok Lee
Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Korea
Correspondence
Dong Seok Lee, Department of Biomedical Laboratory Science, Inje University, 197 Inje-ro, Gimhae, Gyeongnam 50834, Korea.
Email: [email protected]
Search for more papers by this authorJi Hyun Kim,
Gi Suk Nam,
Sung Hyun Kim,
Deok Seon Ryu,
Dong Seok Lee,
Ji Hyun Kim
Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Korea
Search for more papers by this authorGi Suk Nam
Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Korea
Search for more papers by this authorSung Hyun Kim
Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Korea
Search for more papers by this authorDeok Seon Ryu
Department of Biomedical Laboratory Science, Soonchunhyang University, Asan, Korea
Search for more papers by this authorCorresponding Author
Dong Seok Lee
Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae, Korea
Correspondence
Dong Seok Lee, Department of Biomedical Laboratory Science, Inje University, 197 Inje-ro, Gimhae, Gyeongnam 50834, Korea.
Email: [email protected]
Search for more papers by this authorAbstract
Targeted therapy at the molecular level is important for pancreatic cancer treatment. This study looked over the anticancer activity of Orostachys japonicus in a human pancreatic cancer cell line, PANC-1. An ethyl acetate fraction containing quercetin, kaempferol, and flavonol glycosides from O. japonicus (OJE) exhibited significant anticancer activity against the PANC-1. OJE activated caspase-3, caspase-8, and caspase-9, leading to the induction of both intrinsic and extrinsic apoptosis pathways. It also inhibited cyclin D1, cyclin B1, and cyclin-dependent kinase 4, representing cell cycle arrest at both G1/S and G2/M phases. In addition, OJE phosphorylated MAPKs such as p38, JNK, and ERK, which are important upstream signaling factors in apoptosis and arrest of cell cycle inducing system. In conclusion, OJE effectively exerted antipancreatic cancer activity via induction of apoptosis directed by both intrinsic and extrinsic pathways and arrest of cell cycle regulated at both G1/S and G2/M stages, which is activated by MAPKs, p38, JNK, and ERK.
CONFLICT OF INTEREST
The authors declare no conflict of interest.
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