Fast synthesis and bioconjugation of 68Ga core-doped extremely small iron oxide nanoparticles for PET/MR imaging
Juan Pellico
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Universidad Complutense de Madrid and CIBERES, 28040 Madrid, Spain
Search for more papers by this authorJesús Ruiz-Cabello
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Universidad Complutense de Madrid and CIBERES, 28040 Madrid, Spain
Search for more papers by this authorMarina Saiz-Alía
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorGilberto del Rosario
Technological Support Center (CAT), Universidad Rey Juan Carlos, Móstoles, Spain
Search for more papers by this authorSergio Caja
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorMaría Montoya
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorLaura Fernández de Manuel
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorM. Puerto Morales
Departamento de Biomateriales y Materiales Bioinspirados, Instituto de Ciencia de Materiales de Madrid, CSIC, Madrid, Spain
Search for more papers by this authorLucia Gutiérrez
Departamento de Biomateriales y Materiales Bioinspirados, Instituto de Ciencia de Materiales de Madrid, CSIC, Madrid, Spain
Search for more papers by this authorBeatriz Galiana
Physics Department, Universidad Carlos III, Av de la Universidad 40, 28911, Leganés, Madrid, Spain
Search for more papers by this authorJose A. Enríquez
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorCorresponding Author
Fernando Herranz
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Correspondence to: F. Herranz, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC). C/ Melchor Fernández-Almagro 3. 28029 Madrid, Spain. E-mail: [email protected]Search for more papers by this authorJuan Pellico
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Universidad Complutense de Madrid and CIBERES, 28040 Madrid, Spain
Search for more papers by this authorJesús Ruiz-Cabello
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Universidad Complutense de Madrid and CIBERES, 28040 Madrid, Spain
Search for more papers by this authorMarina Saiz-Alía
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorGilberto del Rosario
Technological Support Center (CAT), Universidad Rey Juan Carlos, Móstoles, Spain
Search for more papers by this authorSergio Caja
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorMaría Montoya
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorLaura Fernández de Manuel
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorM. Puerto Morales
Departamento de Biomateriales y Materiales Bioinspirados, Instituto de Ciencia de Materiales de Madrid, CSIC, Madrid, Spain
Search for more papers by this authorLucia Gutiérrez
Departamento de Biomateriales y Materiales Bioinspirados, Instituto de Ciencia de Materiales de Madrid, CSIC, Madrid, Spain
Search for more papers by this authorBeatriz Galiana
Physics Department, Universidad Carlos III, Av de la Universidad 40, 28911, Leganés, Madrid, Spain
Search for more papers by this authorJose A. Enríquez
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Search for more papers by this authorCorresponding Author
Fernando Herranz
Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), C/ Melchor Fernández-Almagro 3, 28029 Madrid, Spain
Correspondence to: F. Herranz, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC). C/ Melchor Fernández-Almagro 3. 28029 Madrid, Spain. E-mail: [email protected]Search for more papers by this authorAbstract
Combination of complementary imaging techniques, like hybrid PET/MRI, allows protocols to be developed that exploit the best features of both. In order to get the best of these combinations the use of dual probes is highly desirable. On this sense the combination of biocompatible iron oxide nanoparticles and 68Ga isotope is a powerful development for the new generation of hybrid systems and multimodality approaches. Our objective was the synthesis and application of a chelator-free 68Ga-iron oxide nanotracer with improved stability, radiolabeling yield and in vivo performance in dual PET/MRI. We carried out the core doping of iron oxide nanoparticles, without the use of any chelator, by a microwave-driven protocol. The synthesis allowed the production of extremely small (2.5 nm) 68Ga core-doped iron oxide nanoparticles. The microwave approach allowed an extremely fast synthesis with a 90% radiolabeling yield and T1 contrast in MRI. With the same microwave approach the nano-radiotracer was functionalized in a fast and efficient way. We finally evaluated these dual targeting nanoparticles in an angiogenesis murine model by PET/MR imaging. Copyright © 2016 John Wiley & Sons, Ltd.
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