Regulating CRISPR/Cas9 Using Streptavidin-Biotin Interactions†
Wei Shen
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
These authors contribute to this work equally.
†Dedicated to the 130th Anniversary of Wuhan University.
Search for more papers by this authorWei Xiong
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
These authors contribute to this work equally.
†Dedicated to the 130th Anniversary of Wuhan University.
Search for more papers by this authorQianqian Qi
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
These authors contribute to this work equally.
†Dedicated to the 130th Anniversary of Wuhan University.
Search for more papers by this authorXingyu Liu
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorZhongpao Xie
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorYuanyuan Zhang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorCorresponding Author
Jinxuan Hou
Department of Thyroid & Breast Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071 China
E-mail: [email protected], [email protected]Search for more papers by this authorCorresponding Author
Tian Tian
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
E-mail: [email protected], [email protected]Search for more papers by this authorXiang Zhou
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorWei Shen
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
These authors contribute to this work equally.
†Dedicated to the 130th Anniversary of Wuhan University.
Search for more papers by this authorWei Xiong
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
These authors contribute to this work equally.
†Dedicated to the 130th Anniversary of Wuhan University.
Search for more papers by this authorQianqian Qi
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
These authors contribute to this work equally.
†Dedicated to the 130th Anniversary of Wuhan University.
Search for more papers by this authorXingyu Liu
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorZhongpao Xie
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorYuanyuan Zhang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorCorresponding Author
Jinxuan Hou
Department of Thyroid & Breast Surgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071 China
E-mail: [email protected], [email protected]Search for more papers by this authorCorresponding Author
Tian Tian
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
E-mail: [email protected], [email protected]Search for more papers by this authorXiang Zhou
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorComprehensive Summary
Currently, CRISPR/Cas9 technology has found widespread applications across various domains. However, the utility of CRISPR/Cas9 is encumbered by issues pertaining to its reliability and safety, primarily stemming from the uncontrolled activity of the system. Therefore, the design and development of CRISPR/Cas9 systems with controllable activity is of paramount importance. Biotin, characterized by its small molecular weight, and streptavidin, distinguished by its substantial spatial steric hindrance, can be harnessed as an ideal OFF switch (termed a "bioactivity brake") due to their interaction characteristics. In this work, we present a strategy that employs the streptavidin-biotin interaction as a "brake system" for CRISPR/Cas9, effectively allowing for the shutdown of the enzymatic activity of CRISPR/Cas9.
Supporting Information
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Appendix S1: Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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