A Label-Free Deoxyribozyme Sensor for m6A Demethylase Activity Detection†
Xiuyuan Wang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorShusheng Yu
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorYuqiu He
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorFuan Wang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorCorresponding Author
Xiaoqing Liu
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
E-mail: [email protected]Search for more papers by this authorXiuyuan Wang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorShusheng Yu
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorYuqiu He
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorFuan Wang
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
Search for more papers by this authorCorresponding Author
Xiaoqing Liu
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072 China
E-mail: [email protected]Search for more papers by this authorDedicated to the 130th Anniversary of Wuhan University.
Comprehensive Summary
A label-free deoxyribozyme (DNAzyme) biosensor for m6A demethylase activity detection is developed. When demethylase FTO (Fat mass and obesity-associated protein), an important m6A demethylase of ALKBH demethylase family is present, the m6A group is specifically demethylated, and the cleavage activity of DNAzyme is restored. This allows the cleavage product that contains G-quadruplex sequence to bind to thioflavin T (ThT) and generate fluorescence signals. The biosensor shows high specificity and sensitivity, and fast reaction speed. Our study demonstrates a new design of allosteric DNAzyme for sensing. This method represents the first label-free nucleic acid biosensor for FTO assay, providing a feasible route towards inhibitors screening.
Supporting Information
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Appendix S1: Supporting Information |
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