Optimization of Culture Conditions of Immobilized Cells for Enzyme Excretion and Cell Lysis
Corresponding Author
Rohaida Che Man
Universiti Malaysia Pahang, Department of Chemical Engineering, College of Engineering, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Correspondence: Rohaida Che Man ([email protected]), Department of Chemical Engineering, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia.Search for more papers by this authorRosli Md Illias
Universiti Teknologi Malaysia, School of Chemical and Energy Engineering, Faculty of Engineering, 81310 Skudai, Johor, Malaysia
Search for more papers by this authorAizi Nor Mazila Ramli
Universiti Malaysia Pahang, College of Computing and Applied Sciences, Faculty of Industrial Sciences and Technology, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Search for more papers by this authorSiti Kholijah Abdul Mudalip
Universiti Malaysia Pahang, Department of Chemical Engineering, College of Engineering, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Universiti Malaysia Pahang, Centre of Excellence for Advanced Research in Fluid Flow (CARIFF), Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Search for more papers by this authorCorresponding Author
Rohaida Che Man
Universiti Malaysia Pahang, Department of Chemical Engineering, College of Engineering, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Correspondence: Rohaida Che Man ([email protected]), Department of Chemical Engineering, College of Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia.Search for more papers by this authorRosli Md Illias
Universiti Teknologi Malaysia, School of Chemical and Energy Engineering, Faculty of Engineering, 81310 Skudai, Johor, Malaysia
Search for more papers by this authorAizi Nor Mazila Ramli
Universiti Malaysia Pahang, College of Computing and Applied Sciences, Faculty of Industrial Sciences and Technology, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Search for more papers by this authorSiti Kholijah Abdul Mudalip
Universiti Malaysia Pahang, Department of Chemical Engineering, College of Engineering, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Universiti Malaysia Pahang, Centre of Excellence for Advanced Research in Fluid Flow (CARIFF), Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Search for more papers by this authorAbstract
Recombinant enzyme excretion is essential due to stable and active protein production. However, enzyme excretion, especially in the Escherichia coli system, may increase the occurrence of cell lysis. Thus, cell immobilization was used, and optimization of culture conditions was conducted to increase enzyme excretion and limit cell lysis. The culture conditions were optimized using a response surface methodology. CGTase excretion and cell lysis were analyzed by CGTase and β-galactosidase assays, respectively. High CGTase excretion with low cell lysis was achieved after the optimization process. The results suggest that optimization of the cell immobilization system increased CGTase excretion with low cell lysis and made it more valuable for industrial applications.
References
- 1 J. R. López-Menchero, M. Ogawa, J. C. Mauricio, J. Moreno, J. Moreno-García, LWT – Food Sci Technol. 2021, 144, 111250. DOI: https://doi.org/10.1016/j.lwt.2021.111250
- 2 H. Moon, S. Kim, B. H. Jo, H. J. Cha, J. CO2 Util. 2020, 39, 101172. DOI: https://doi.org/10.1016/j.jcou.2020.101172
- 3 C. Mazzer, L. R. Ferreira, J. R. T. Rodella, C. Moriwaki, G. Matioli, Biochem. Eng. J. 2008, 41, 79–86. DOI: https://doi.org/10.1016/j.bej.2008.03.010
- 4 J. Żur, A. Piński, J. Michalska, K. Hupert-Kocurek, A. Nowak, D. Wojcieszyńska, U. Guzik, Int. Biodeterior. Biodegrad. 2020, 149, 104919. DOI: https://doi.org/10.1016/j.ibiod.2020.104919
- 5 G. Zhou, L. Fu, X. Li, Food Chem. 2015, 170, 186–192. DOI: https://doi.org/10.1016/j.foodchem.2014.08.080
- 6 W. Ye, X. Wang, Y. Liu, J. Chen, Appl. Therm. Eng. 2021, 188, 116557. DOI: https://doi.org/10.1016/j.applthermaleng.2021.116557
- 7 Q. Li, D. An, J. Feng, J. Hu, W. Cao, L. Guo, Int. J. Hydrogen Energy 2021, 46, 6310–6320. DOI: https://doi.org/10.1016/j.ijhydene.2020.11.124
- 8 P. Vijayaraghavan, M. V. Arasu, R. Anantha Rajan, N. A. Al-Dhabi, Saudi J. Biol. Sci. 2019, 26, 217–224. DOI: https://doi.org/10.1016/j.sjbs.2018.08.029
- 9 M. A. Jonet, N. M. Mahadi, A. M. A. Murad, A. Rabu, F. D. A. Bakar, R. A. Rahim, K. O. Low, R. M. Illias, J. Mol. Microbiol. Biotechnol. 2012, 22, 48–58. DOI: https://doi.org/10.1159/000336524
- 10 R. C. Man, A. F. Ismail, N. F. Ghazali, S. F. Z. M. Fuzi, R. M. Illias, Biochem. Eng. J. 2015, 98, 91–98. DOI: https://doi.org/10.1016/j.bej.2015.02.013
- 11 R. C. Man, A. F. Ismail, S. F. Z. M. Fuzi, N. F. Ghazali, R. M. Illias, Process Biochem. 2016, 51, 474–483. DOI: https://doi.org/10.1016/j.procbio.2016.01.002
- 12 H. K. Sian, M. Said, O. Hassan, K. Kamaruddin, A. F. Ismail, R. A. Rahman, N. A. N. Mahmood, R. M. Illias, Process Biochem. 2005, 40, 1101–1111. DOI: https://doi.org/10.1016/j.procbio.2004.03.018
- 13 K. Einsfeldt, J. B. S. Júnior, A. P. C. Argondizzo, M. A. Medeiros, T. L. M. Alves, R. V. Almeida, A. L. Larentis, Vaccine 2011, 29, 7136–7143. DOI: https://doi.org/10.1016/j.vaccine.2011.05.073
- 14 M. Gadgil, V. Kapur, W. Hu, Biotechnol. Prog. 2005, 21, 689–699. DOI: https://doi.org/10.1021/bp049630l
- 15 A. Najjari, H. Shahbazmohammadi, E. Omidinia, Biocatal. Agric. Biotechnol. 2021, 32, 101943. DOI: https://doi.org/10.1016/j.bcab.2021.101943
- 16 H. H. Su, R. Y. Xu, Z. D. Yang, Y. S. Guo, J. Y. Gao, L. Z. Mo, Y. F. Gao, H. Cheng, P. J. Zhang, J. S. Huang, LWT 2021, 143, 111054. DOI: https://doi.org/10.1016/j.lwt.2021.111054
- 17 B. R. Glick, Biotechnol. Adv. 1995, 13, 247–261. DOI: https://doi.org/10.1016/0734-9750(95)00004-a
- 18 N. H. Abd Rahman, J. M. Jahim, M. S. Abdul Munaim, R. A. Rahman, S. F. Z. Fuzi, R. M. Illias, Enzyme Microb. Technol. 2020, 135, 109495. DOI: https://doi.org/10.1016/j.enzmictec.2019.109495
- 19 C. H. Schein, M. H. M. Noteborn, Bio/Technology 1988, 6, 291–294. DOI: https://doi.org/10.1038/nbt0388-291
- 20 Z. F. Li, B. Li, Z. G. Liu, M. Wang, Z. B. Gu, G. C. Du, J. Wu, J. Chen, J. Agric. Food Chem. 2009, 57, 6231–6237. DOI: https://doi.org/10.1021/jf901239k
- 21 H. M. Yu, H. Luo, H. Zhou, J. Hou, Z. Y. Shen, J. Mol. Catal. B: Enzym. 2006, 43, 118–123. DOI: https://doi.org/10.1016/j.molcatb.2006.07.002
