Untargeted Metabolomic to Access Chemical Differences Induced by Dual Endophyte Cultures Isolated from Euphorbia Umbellata
Amanda Santos Gusmão
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Formal analysis (equal), Investigation (lead), Methodology (lead)
Search for more papers by this authorJoão Carlos Silva Conceição
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Formal analysis (equal), Investigation (equal)
Search for more papers by this authorStefane Moreira de Queiros Santos
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Investigation (equal), Methodology (equal)
Search for more papers by this authorCecilia Lima Sampaio Pereira
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Investigation (equal)
Search for more papers by this authorDouglas Siqueira de Almeida Chaves
Pharmaceutical Science Department, Health and Biological Science Institute, Federal Rural University of Rio de Janeiro, Seropédica, 23897-000 Brazil
Contribution: Formal analysis (supporting), Investigation (supporting), Writing - review & editing (equal)
Search for more papers by this authorKaren de Jesus Nicácio
Department of Chemistry, Federal University of Mato Grosso, Cuiabá, 14040-901 Brazil
Contribution: Formal analysis (equal), Methodology (lead), Validation (lead), Writing - review & editing (equal)
Search for more papers by this authorDaniela Aparecida Chagas-Paula
Chemistry Institute, Federal University of Alfenas, Alfenas, 37130-001 Brazil
Contribution: Conceptualization (equal), Funding acquisition (lead), Writing - review & editing (lead)
Search for more papers by this authorCorresponding Author
Eliane de Oliveira Silva
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Conceptualization (lead), Formal analysis (lead), Project administration (lead), Supervision (lead), Writing - original draft (lead)
Search for more papers by this authorAmanda Santos Gusmão
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Formal analysis (equal), Investigation (lead), Methodology (lead)
Search for more papers by this authorJoão Carlos Silva Conceição
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Formal analysis (equal), Investigation (equal)
Search for more papers by this authorStefane Moreira de Queiros Santos
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Investigation (equal), Methodology (equal)
Search for more papers by this authorCecilia Lima Sampaio Pereira
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Investigation (equal)
Search for more papers by this authorDouglas Siqueira de Almeida Chaves
Pharmaceutical Science Department, Health and Biological Science Institute, Federal Rural University of Rio de Janeiro, Seropédica, 23897-000 Brazil
Contribution: Formal analysis (supporting), Investigation (supporting), Writing - review & editing (equal)
Search for more papers by this authorKaren de Jesus Nicácio
Department of Chemistry, Federal University of Mato Grosso, Cuiabá, 14040-901 Brazil
Contribution: Formal analysis (equal), Methodology (lead), Validation (lead), Writing - review & editing (equal)
Search for more papers by this authorDaniela Aparecida Chagas-Paula
Chemistry Institute, Federal University of Alfenas, Alfenas, 37130-001 Brazil
Contribution: Conceptualization (equal), Funding acquisition (lead), Writing - review & editing (lead)
Search for more papers by this authorCorresponding Author
Eliane de Oliveira Silva
Departament of Organic Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, 40170-115 Brazil
Contribution: Conceptualization (lead), Formal analysis (lead), Project administration (lead), Supervision (lead), Writing - original draft (lead)
Search for more papers by this authorAbstract
Endophytic fungi live asymptomatically inside vegetal tissues, and such uncommon habitat contributes to their exceptional chemical diversity. Isolating natural products from endophytic fungi could fail due to silent biosynthetic gene clusters under ordinary in vitro culture conditions, and co-culturing has been assayed to trigger their metabolism. We carried out single and dual cultures with 13 endophyte strains isolated from Euphorbia umbellata leaves. Multivariate statistics applied to untargeted metabolomics compared the chemical profiles of all endophyte cultures. PCA analysis guided the selection of the Aspergillus pseudonomiae J1 – Porogramme brasiliensis J9 dual culture for its most significant chemical differentiation: Five compounds were putatively annotated in the J1−J9 culture according to UHPLC-HRMS data, kojic acid, haliclonol and its diastereoisomer, caffeic acid, and 2-(3,4-dihydroxyphenyl)acetaldehyde. Analysis by PLS-DA using VIP score showed that kojic acid displayed the most significative importance in discriminating single and dual J1−J9 cultures.
Graphical Abstract
Conflict of interests
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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