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Chemical Composition and Insecticidal Activity of Crithmum Maritimum L. Essential Oil against Stored-Product Beetle Tribolium Castaneum
Mayssa Ben Mustapha,
Afifa Zardi-Bergaoui,
Ikbel Chaieb,
Guido Flamini,
Roberta Ascrizzi,
Hichem Ben Jannet,
Mayssa Ben Mustapha
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Team: Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
Search for more papers by this authorAfifa Zardi-Bergaoui
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Team: Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
Search for more papers by this authorIkbel Chaieb
University of Sousse, Regional Center of Research on Horticulture and Organic Agriculture, 57 Chott Mariem, TN-4042 Sousse, Tunisia
Search for more papers by this authorGuido Flamini
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 33, 56126 Pisa, Italy
Centro Interdipartimentale di Ricerca ‘Nutraceutica e Alimentazione per la Salute' Nutrafood, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy
Search for more papers by this authorRoberta Ascrizzi
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 33, 56126 Pisa, Italy
Search for more papers by this authorCorresponding Author
Hichem Ben Jannet
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Team: Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
Search for more papers by this authorMayssa Ben Mustapha,
Afifa Zardi-Bergaoui,
Ikbel Chaieb,
Guido Flamini,
Roberta Ascrizzi,
Hichem Ben Jannet,
Mayssa Ben Mustapha
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Team: Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
Search for more papers by this authorAfifa Zardi-Bergaoui
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Team: Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
Search for more papers by this authorIkbel Chaieb
University of Sousse, Regional Center of Research on Horticulture and Organic Agriculture, 57 Chott Mariem, TN-4042 Sousse, Tunisia
Search for more papers by this authorGuido Flamini
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 33, 56126 Pisa, Italy
Centro Interdipartimentale di Ricerca ‘Nutraceutica e Alimentazione per la Salute' Nutrafood, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy
Search for more papers by this authorRoberta Ascrizzi
Dipartimento di Farmacia, Università di Pisa, Via Bonanno 33, 56126 Pisa, Italy
Search for more papers by this authorCorresponding Author
Hichem Ben Jannet
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Team: Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia
Search for more papers by this authorAbstract
Several plant essential oils have been used against diverse insect pests since, unlike conventional pesticides, they pose almost no risk to humans and the environment. For this reason, the essential oil (EO) isolated from the fresh leaves of Crithmum maritimum L. and its fractions (F1–F5) obtained by chromatographic simplification were investigated for their chemical profile, as well as for their toxicity and repellency effects against Tribolium castaneum (Herbst) adults. The analysis by GC/MS allowed the identification of 92.8–99.1 % of the compositions of the total oil (EO) and of its fractions (F1–F5). The EO and its fractions F3–F5 were characterized by the presence of a high amount of phenylpropanoids (94.4, 94.8, 93.6, and 88.7 %, respectively): in all the samples, dill apiole was the most abundant component (EO: 94.1 %, F3: 94.6 %, F4: 93.4 %, and F5: 83.3 %). In addition, the repellency assay results showed that the volatile fraction F5 and the complete EO exhibited a higher repellency towards T. castaneum (97 % and 93 %, respectively) after 2 h of exposure at the dose of 0.04 μL/cm2. The median lethal dose of the topical application of the EO was 9 %. Furthermore, the fraction F1 possessed interesting contact toxicity against T. castaneum (80 % of mortality) at the concentration of 10 %. These results suggested that the essential oil of C. maritimum leaves might be used as an alternative to synthetic insecticides in order to prevent insects from damaging the stored products.
Graphical Abstract
References
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