Volume 35, Issue 12 e6435

RESEARCH ARTICLE

Synthesis and morphological studies of Tc-99m-labeled lupulone-conjugated Fe₃O₄@TiO₂ nanocomposite, and in vitro cytotoxicity activity on prostate cancer cell lines

Elif Tutun,

Elif Tutun

orcid.org/0000-0002-0995-1107

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Izmir, Turkey

Contribution: Investigation (equal), Methodology (equal)

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Volkan Tekin,

Volkan Tekin

orcid.org/0000-0002-0264-3814

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Izmir, Turkey

Contribution: Methodology (equal), Visualization (equal)

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Volkan Yasakcı,

Volkan Yasakcı

orcid.org/0000-0002-4133-3886

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Izmir, Turkey

Contribution: Methodology (equal)

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Ömer Aras,

Ömer Aras

orcid.org/0000-0003-1758-0542

Memorial Sloan Kettering Cancer Center, New York, New York, USA

Contribution: Methodology (equal), Validation (equal), Visualization (equal)

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Perihan Ünak,

Corresponding Author

Perihan Ünak

[email protected]

orcid.org/0000-0002-5464-2987

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Izmir, Turkey

Correspondence

Perihan Ünak, Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Turkey.

Email: [email protected]

Contribution: Conceptualization (lead), Data curation (lead), Investigation (lead), Methodology (lead), Project administration (equal), Resources (equal), Supervision (equal)

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Elif Tutun,

Elif Tutun

orcid.org/0000-0002-0995-1107

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Izmir, Turkey

Contribution: Investigation (equal), Methodology (equal)

Search for more papers by this author

Volkan Tekin,

Volkan Tekin

orcid.org/0000-0002-0264-3814

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Izmir, Turkey

Contribution: Methodology (equal), Visualization (equal)

Search for more papers by this author

Volkan Yasakcı,

Volkan Yasakcı

orcid.org/0000-0002-4133-3886

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Izmir, Turkey

Contribution: Methodology (equal)

Search for more papers by this author

Ömer Aras,

Ömer Aras

orcid.org/0000-0003-1758-0542

Memorial Sloan Kettering Cancer Center, New York, New York, USA

Contribution: Methodology (equal), Validation (equal), Visualization (equal)

Search for more papers by this author

Perihan Ünak,

Corresponding Author

Perihan Ünak

[email protected]

orcid.org/0000-0002-5464-2987

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Izmir, Turkey

Correspondence

Perihan Ünak, Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Turkey.

Email: [email protected]

Contribution: Conceptualization (lead), Data curation (lead), Investigation (lead), Methodology (lead), Project administration (equal), Resources (equal), Supervision (equal)

Search for more papers by this author

First published: 02 September 2021

https://doi.org/10.1002/aoc.6435

Citations: 3

Funding information: National Cancer Institute, Grant/Award Number: P30 CA008748

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Abstract

The purpose of this study was to develop a multifunctional theranostic probe for imaging (magnetic resonance imaging [MRI] and single-photon emission computed tomography [SPECT]) and therapy (photodynamic therapy). For this purpose, Tc-99m-labeled lupulone-conjugated Fe₃O₄@TiO₂ nanocomposites (^99mTc-DTPA-Fe₃O₄@TiO₂-HLP and ^99mTc-DTPA-Fe₃O₄@TiO₂-ALP nanocomposites) were synthesized. The average diameter of the nanocomposites was 171 ± 20 nm as seen on transmission electron microscopy images. Fe₃O₄@TiO₂ nanocomposites exhibited fluorescence spectra at an emission wavelength of 314 nm. Lupulone-conjugated Fe₃O₄@TiO₂ nanocomposites were spherical-shaped with a suitable dispersion and without visible aggregation, and their radiolabeling yields were over 85%. Healthy (RWPE-1 normal human prostate epithelial cell line) and cancer prostate cell lines (PC-3 human prostate cancer cell line) were used to determine the in vitro biological behavior of the nanocomposites. The PC-3 cells treated with lupulone-conjugated Fe₃O₄@TiO₂ nanocomposites showed a lower cell viability compared with RWPE-1 cells treated with lupulone-conjugated Fe₃O₄@TiO₂ nanocomposites. Lupulone-modified Fe₃O₄@TiO₂ nanocomposites may serve in the future as a multifunctional probe for positron emission tomography (PET)/MRI, photodynamic therapy, and hyperthermia therapy of cancer.

Open Research

DATA AVAILABILITY STATEMENT

Data are available on request from the authors.

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Volume35, Issue12

December 2021

e6435

Synthesis and morphological studies of Tc-99m-labeled lupulone-conjugated Fe₃O₄@TiO₂ nanocomposite, and in vitro cytotoxicity activity on prostate cancer cell lines

Abstract

Open Research

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REFERENCES

Citing Literature

References

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Synthesis and morphological studies of Tc-99m-labeled lupulone-conjugated Fe3O4@TiO2 nanocomposite, and in vitro cytotoxicity activity on prostate cancer cell lines

Abstract

Open Research

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Synthesis and morphological studies of Tc-99m-labeled lupulone-conjugated Fe₃O₄@TiO₂ nanocomposite, and in vitro cytotoxicity activity on prostate cancer cell lines