Volume 64, Issue 30 e202507787

Research Article

Intermediate Layer-Engineered Lanthanide Nanoparticles Enable Deep Bioorthogonal Liver Tumor and Vascular Imaging via Switchable NIR-II Emissions

Kehong Lv

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

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Hongxia Yue,

Hongxia Yue

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

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Chunyan Li,

Chunyan Li

CAS Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory of Functional Molecular Imaging Technology Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123 P.R. China

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Shengzhe Chen,

Shengzhe Chen

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

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Hongli Wang,

Hongli Wang

College of Animal Science, Jilin University, Changchun, 130012 P.R. China

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Ruohao Zhang,

Ruohao Zhang

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

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Jing Feng,

Corresponding Author

Jing Feng

[email protected]

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

E-mail: [email protected], [email protected]

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Hongjie Zhang,

Corresponding Author

Hongjie Zhang

[email protected]

orcid.org/0000-0001-5433-8611

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

Department of Chemistry, Tsinghua University, Beijing, 100084 P.R. China

E-mail: [email protected], [email protected]

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Kehong Lv,

Kehong Lv

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

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Hongxia Yue,

Hongxia Yue

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

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Chunyan Li,

Chunyan Li

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Shengzhe Chen,

Shengzhe Chen

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

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Hongli Wang,

Hongli Wang

College of Animal Science, Jilin University, Changchun, 130012 P.R. China

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Ruohao Zhang,

Ruohao Zhang

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

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Jing Feng,

Corresponding Author

Jing Feng

[email protected]

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

E-mail: [email protected], [email protected]

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Hongjie Zhang,

Corresponding Author

Hongjie Zhang

[email protected]

orcid.org/0000-0001-5433-8611

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 P.R. China

School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026 P.R. China

Department of Chemistry, Tsinghua University, Beijing, 100084 P.R. China

E-mail: [email protected], [email protected]

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First published: 23 May 2025

https://doi.org/10.1002/anie.202507787

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Graphical Abstract

By engineering the intermediate layer of lanthanide nanoparticles, we achieve switchable NIR-II emissions, enabling high-resolution vascular imaging at 1525 nm and deep bioorthogonal liver tumor imaging at 1064 nm. This will provide reference for designing imaging probes to adapt to fluorescence imaging in different scenes.

Abstract

The second near-infrared window (NIR-II) has become an attractive optical region for fluorescence imaging. However, due to the complexity in vivo, NIR-II light with various wavelengths for different imaging scenes has not been investigated. Here, we found that 1525 nm light is suitable for high-resolution fluorescence imaging due to low background interference, while its attenuation in vivo makes it unsuitable for deep imaging. The 1064 nm light is suitable for deep imaging. The impact of Yb³⁺ ions and the proportion of the intermediate layer in lanthanide nanoparticles on the modulation of emissions were investigated. The intense 1064 nm emission is achievable when the Yb³⁺ content is 80% and the intermediate layer proportion is 0.1. The bioorthogonal orthotopic liver tumor imaging could be achieved by modifying the lanthanide nanoparticles with DBCO-PEG₂₀₀₀-DSPE and establishing artificial receptors by N-azidoacetylmannosamine-tetraacylated (Ac₄ManNAz). Lanthanide nanoparticles with the intermediate layer proportion of 0.66 and 80% Yb³⁺ facilitate intense 1525 nm emission, enabling the vascular imaging. Based on the imaging characteristics of NIR-II emissions with different wavelengths in vivo, this work achieves high-resolution imaging and deep imaging through intermediate layer regulation of lanthanide nanoparticles, providing an opportunity to explore a new generation of high-performance fluorescent probes.

Conflict of Interests

The authors declare no conflict of interest.

Open Research

Data Availability Statement

The data that support the findings of this study are available on request from the corresponding author.

Supporting Information

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Volume64, Issue30

July 21, 2025

e202507787

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Hot Topic: Bioorthogonal Chemistry

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Intermediate Layer-Engineered Lanthanide Nanoparticles Enable Deep Bioorthogonal Liver Tumor and Vascular Imaging via Switchable NIR-II Emissions

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Abstract

Conflict of Interests

Open Research

Data Availability Statement

Supporting Information

References

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Intermediate Layer-Engineered Lanthanide Nanoparticles Enable Deep Bioorthogonal Liver Tumor and Vascular Imaging via Switchable NIR-II Emissions

Graphical Abstract

Abstract

Conflict of Interests

Open Research

Data Availability Statement

Supporting Information

References

References

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